Hepatic Differentiation of Murine Disease-Specific Induced Pluripotent Stem Cells Allows Disease Modelling In Vitro
Figure 3
Hepatic differentiation of toxic milk iPS cells. (a, b) Toxic-milk iPS were subjected to hepatic differentiation using hanging drop method and transduced with lentiviral Alb-GFP expression vector. (c) qRT PCR analysis for hepatic marker genes Afp, Alb, Ck18, Abcc2, Ttr, and Hnf4α. Undifferentiated cells served as negative control, in which no hepatic gene expression was determined until cycle 45 (). (d) During the last seven days of differentiation, the cells were challenged with copper. The toxic-milk mice-derived cells could not export copper, and, therefore, no enrichment of Alb-positive cells was achieved. However, C3H-derived control cells were able to “detoxify” copper after hepatic specifications and, therefore, Alb-eGFP-positive cells were enriched.
