DMSO-Free Programmed Cryopreservation of Fully Dissociated and Adherent Human Induced Pluripotent Stem Cells
Figure 1
Toxicity of 4 cryoprotective agents. The cells were incubated one hour at 37°C in 10% solutions of four CPAs: dimethyl sulfoxide (DMSO), ethylene glycol (EG), propylene glycol (PG), and glycerol (GLY). Recovery of the Calcein-PM+/7AAD-cells in comparison with control (QUANTA allows precise measurement of the cell concentration that was converted into total cell yield). The cells were then detached and plated according to the routine procedure, and on day 2, before the first change of the medium, the unattached cells were also harvested. The average attachment efficiency of the untreated control was in a range 65–77%, and practically 95% of the attached iPS cells were Nanog positive after 2 days of incubation (measured by using software imaging). The actual experimental results (refered to as “iPSC Recovery”) normalized to the control value are depicted in Figure 3 (group “TOX”). The different letters indicate statistically significant difference among the two CPAs. , .
