Retinal Pigment Epithelium and Müller Progenitor Cell Interaction Increase Müller Progenitor Cell Expression of PDGFR𝛼 and Ability to Induce Proliferative Vitreoretinopathy in a Rabbit Model

Figure 2

Western blot analysis of coculture samples at days 1, 3, 5, and 7 shows noticeable increases in PDGFR𝛼 expression in MIO-M1 MPCs. Transfected ARPE19 cells with increased expression of PDGFR𝛼 (RPE𝛼) are used as control for comparison. PDGFR𝛽 levels remain relatively low in both cell types throughout the course of the experiment, with a small increase in MIO-M1 MPCs on day 5 and a more significant increase by day 7. MIO-M1 cells show a small but measurable and consistent decrease in expression of GFAP between days 1 and 7 of the experiment. RPE𝛼 cells are included for control. Protein concentrations were standardized by Bradford assay. Band intensity was standardized by 𝛽-Actin expression (shown). Relative expression ratios are shown.