Feline Neural Progenitor Cells I: Long-Term Expansion under Defined Culture Conditions
Figure 4
Comparison by qPCR of gene expression profile for cNPCs in different culture media. The cNPCs were cultured in either SM or UM for 1 month prior to testing. (a) heat map display showing relative expression levels of 23 genes expressed by NPCs or related progeny of neural lineage. The scale to the right shows that moderate expression level (as determined by CT value) is shown as black, while lower expression appears increasingly green, and higher expression increasingly red. Viewed in this way, the general similarity of the 2 conditions is evident in that the color of a particular gene tends to be conserved across conditions, even if the intensity often varies. (b) fold change, with SM used as calibrator. Note that expression is represented on a log2 scale, such that 1.00 corresponds to a 2 fold change. Viewed in this way, differences in expression are highlighted. The majority of genes showed less than 2 fold change (between 1.00 to −1.00, on log2 scale), again confirming the general similarity between conditions; however, a number of individual genes fell outside this range. The error bars-show standard deviation.
