Ex Vivo Expansion of Human Mesenchymal Stem Cells in Defined Serum-Free Media
Figure 4
An overview demonstrating a process for the development of a defined serum-free medium for hMSCs. (a) To replace ill-defined serum with defined supplements, a variety of medium constituents, including basal media, additional nutrients (e.g., lipids and vitamins), binding proteins, physiochemical reagents (e.g., buffer), hormones, growth factors, and attachment factors were selected. (b) A sequential strategy was designed for screening effectively the selected basal media and medium additives to develop a defined serum-free condition that supports the isolation and expansion of hMSCs [76].
