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Stem Cells International
Volume 2012, Article ID 128732, 11 pages
Research Article

Adult-Brain-Derived Neural Stem Cells Grafting into a Vein Bridge Increases Postlesional Recovery and Regeneration in a Peripheral Nerve of Adult Pig

1Unité de Chirurgie et Physiologie Expérimentale (UCPE), Institut de Médecine Tropicale, 58 boulevard Charles Livon, 13007 Marseille, France
2Unité Physiologie de la Reproduction et des Comportements, UMR 85, Centre INRA de Tours, 37380 Nouzilly, France
3Laboratoire d'Ecologie fonctionnelle, Bâtiment 4R3, 118 route de Narbonne, 31062 Toulouse cedex 9, France
4Unité de Rétrovirologie, U421 INSERM, Ecole Normale Supérieure de Lyon, 46 Allée d'Italie, 69364 Lyon cedex 07, France

Received 15 August 2011; Revised 6 October 2011; Accepted 7 October 2011

Academic Editor: Henry J. Klassen

Copyright © 2012 Olivier Liard et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We attempted transplantation of adult neural stem cells (ANSCs) inside an autologous venous graft following surgical transsection of nervis cruralis with 30 mm long gap in adult pig. The transplanted cell suspension was a primary culture of neurospheres from adult pig subventricular zone (SVZ) which had been labeled in vitro with BrdU or lentivirally transferred fluorescent protein. Lesion-induced loss of leg extension on the thigh became definitive in controls but was reversed by 45–90 days after neurosphere-filled vein grafting. Electromyography showed stimulodetection recovery in neurosphere-transplanted pigs but not in controls. Postmortem immunohistochemistry revealed neurosphere-derived cells that survived inside the venous graft from 10 to 240 post-lesion days and all displayed a neuronal phenotype. Newly formed neurons were distributed inside the venous graft along the severed nerve longitudinal axis. Moreover, ANSC transplantation increased CNPase expression, indicating activation of intrinsic Schwann cells. Thus ANSC transplantation inside an autologous venous graft provides an efficient repair strategy.