Effects of Intravenous Administration of Human Umbilical Cord Blood Stem Cells in 3-Acetylpyridine-Lesioned Rats
Figure 2
Immunodetection of human leucocyte antigen-ABC in the brain stem and cerebellum of 3-AP ataxic rats. (a) and (c) show HLA-ABC immunostaining (green) in the ventral (a) and dorsal (c) brain stem of 3-AP-lesioned rats receiving HuUCBMCs grafts. (b) Shows HLA-ABC (b, green) and laminin (b, red) immunoreactivity in the ventral brain stem. Note how HLA-ABC-positive cells are associated to laminin-positive blood vessels (b, yellow, white arrows) or integrated in the parenchyma (c, white arrowheads). In the cerebellum HLA-ABC-positive cells (d–f, green) are located near to the vermis in laminin-positive blood vessels (d, red), and in the parenchyma of the granular (e, green) and molecular layers (f, green, white stars) of the cerebellar cortex. Nuclei were counterstained with Hoechst 33342 (blue). Scale bar: 25 m (b and d), 50 m (c, e, and f), and 100 m (a).
