Effects of Intravenous Administration of Human Umbilical Cord Blood Stem Cells in 3-Acetylpyridine-Lesioned Rats
Figure 5
Immunodetection of glial cells in the inferior olive and cerebellum of 3-AP ataxic rats. (a) and (b) show the histochemical detection of isolectin B4 (IB4, red) in the inferior olive of 3-AP-lesioned rats 48 hours (a) and 10 days (b, c) after lesion. Note how 3-AP lesioned rats that received HuUCBMCs grafts (c, red) show lower IB4 staining at 10 days after lesion than ataxic rats treated with vehicle (b, red). (d) to (g) Show OX6 immunostaining in the inferior olive of naïve rats (d, red) and 3-AP-lesioned rats treated with vehicle (e, red) or HuUCBMCs (f, red). Cell transplantation increases OX6 immunoreactivity (f, red). (g) shows double immunolabeling for OX6 (red) and PCNA (green) in the inferior olive of 3-AP + cells-treated rats. (h) to (j) show GFAP immunoreactivity in the cerebellum. Note how HuUCBMCs grafts reduce GFAP immunolabeling in this structure (j, green). Nuclei were counterstained with Hoechst 33342 (blue). Scale bar: 25 m (g, h, I, and j), 50 m (a, b, c, d, e, and f).
