Table of Contents Author Guidelines Submit a Manuscript
Stem Cells International
Volume 2012, Article ID 263264, 7 pages
Research Article

The Influence of Brightness on Functional Assessment by mfERG: A Study on Scaffolds Used in Retinal Cell Transplantation in Pigs

1Department of Ophthalmology, Glostrup Hospital, Copenhagen University Hospital, Nordre Ringvej 57, 2600 Glostrup, Denmark
2NuVention Solutions Inc., Valley View, OH 44125, USA
3Case Western Reserve University, Cleveland, OH 44106, USA
4Eye Pathology Institute, University of Copenhagen, 2100 Copenhagen, Denmark
5Schepens Eye Research Institute, Department of Ophthalmology, Harvard Medical School, Boston, MA 02114, USA
6The Gavin Herbert Eye Institute and Stem Cell Research Center, University of California, Irvine, CA 92697, USA
7Department of Ophthalmology and Visual Sciences, Kentucky Lions Eye Center, University of Louisville, Louisville, KY 40208, USA

Received 16 August 2011; Accepted 7 November 2011

Academic Editor: Budd A. Tucker

Copyright © 2012 A. T. Christiansen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


To determine the effect of membrane brightness on multifocal electroretinograms (mfERGs), we implanted poly lactic-co-glycolic acid (PLGA) membranes in the subretinal space of 11 porcine eyes. We compared membranes with their native shiny white color with membranes that were stained with a blue dye (Brilliant Blue). Histological and electrophysiological evaluation of the overlying retina was carried out 6 weeks after implantation. Histologically, both white and blue membranes degraded in a spongiform manner leaving a disrupted outer retina with no preserved photoreceptor segments. Multifocal ERG revealed the white membranes to have a significantly higher P1-amplitude ratio than the blue and a correlation between brightness ratio and P1-amplitude ratio was found . Based on our findings, we conclude that bright subretinal objects can produce normal mfERG amplitude ratios even when the adjacent photoreceptors are missing. Functional assessment with mfERG in scaffold implant studies should therefore be evaluated with care.