Cell Reprogramming, IPS Limitations, and Overcoming Strategies in Dental Bioengineering

Figure 1

Basic scheme of tissue engineering. A biopsy is carried out to extract cells from the patient. These can be endogenous organ-specific multipotent SCs, or alternatively they can be adult differentiated somatic cells, reprogrammed to IPS cells. SCs are isolated, expanded and differentiated to the cell type of interest in an appropriate culture medium, seeded in a scaffold, and cultured in vitro. At this point, the new tissue is implanted in the patient.