Human Muscle Progenitor Cells Displayed Immunosuppressive Effect through Galectin-1 and Semaphorin-3A
Figure 1
Human myoblasts characterization and PBMCs proliferation inhibition. (a) Immunophenotyping by flow cytometry analysis: cells were labeled with specific antibodies (red line) or isotype control (green line). (b) Myogenic differentiation ability of CD56+ cells. Myotubes expressed myosin heavy chain (red), nuclei were labeled with DAPI (blue). (c) PBMCs were cultured in the presence of increasing number of myoblasts at ratio ranging from 1/1000 to 1/5. Data are means ± SEM of triplicates from one representative of at least 10 independent experiments. P values from Student’s t-test are indicated (**P < 0.01, ***P < 0.001).
