Human Muscle Progenitor Cells Displayed Immunosuppressive Effect through Galectin-1 and Semaphorin-3A
Figure 2
Human myoblasts inhibit PBMCs proliferation independently of PGE-2 and TGFβ-1. (a) PGE-2 (left panel) and TGF-β (right panel) levels were assessed by ELISA on culture supernatants of MSCs and myoblasts, in the presence (black bars) or absence (white bars) of PBMCs. (b) TGF-β1 blocking antibodies (10 to 40 μg/mL) were used to neutralize the immunosuppressive activity of myoblasts on PBMCs. PBMCs and myoblasts were cultured at a ratio 1/5.
