Induction of Pluripotency in Adult Equine Fibroblasts without c-MYC

Table 1

List of primers used for RT-PCR.

Markers	Primer F	Primer R	References
GAPDH	GATTCCACCCATGGCAAGTTCCATGGCAC	GCATCGAAGGTGGAAGAGTGGGTGTCACT
OCT4	TCT TTC CAC CAG GCC CCC GGC TC	TGC GGG CGG ACA TGG GGA GAT CC
NANOG	TCA AGG ACA GGT TTC AGA AGC A	GCT GGG ATA CTC CAC TGG TG
SOX2	GGT TAC CTC TTC CTC CCA CTC CAG	TTG CCT TAA ACA AGA CCA CGA AA
STAT-3	TCTGGCTAGACAATATCATCGACCTT	TTATTTCCAAACTGCATCAATGAATCT	Li et al. [12]
𝛽-Tubulin III	CAGAGCAAGAACAGCAGCTACTT	GTGAACTCCATCTCGTCCATGCCCTC	Li et al. [12]
GATA-4	CTCTGGAGGCGAGATGGGACGGG	GAGCGGTCATGTAGAGGCCGGCAGGCATT	Li et al. [12]
α-Fetoprotein	CTTACACAAAGAAAGCCCCTCAAC	AAACTCCCAAAGCAGCACGAG	Li et al. [12]
BMP4	TCGTTACCTCAAGGGAGTGG	GGCTTTGGGGATACTGGAAT	Pashaiasl et al. [30]

OCT4 and SOX2 primers were based on primers specific for Homo sapiens primers. The sequences of these genes were blasted against horse nucleotide sequences that have 95% and 94% coverage with the coding sequence of Equus caballus. BMP4 primers were designed on a bovine sequence that has 91% coverage with the coding sequence of Equallus equa. STAT3, GATA4, 𝛽-tubulin III, and α-fetoprotein primers have been applied by Li et al. [12].