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Stem Cells International
Volume 2012, Article ID 452102, 8 pages
http://dx.doi.org/10.1155/2012/452102
Clinical Study

Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate

1The Congenital Heart Institute of Florida (CHIF), Saint Petersburg and Tampa, FL, USA
2Seattle Children’s Hospital Research Institute, University of Washington, 1900 Ninth Ave North, Seattle, WA 98101, USA
3The Congenital Heart Institute of Florida (CHIF), All Children’s Hospital, University of South Florida College of Medicine, Cardiac Surgical Associates of Florida (CSAoF), Saint Petersburg and Tampa, FL, USA
4Department of Pathology and Laboratory Medicine, All Children’s Hospital, Saint Petersburg, FL, USA
5The Congenital Heart Institute of Florida (CHIF), All Children’s Hospital, University of South Florida College of Medicine, Pediatric Cardiology Associates/Pediatrix, Saint Petersburg and Tampa, FL, USA
6Nova Southeastern University, Ft. Lauderdale, FL, USA

Received 19 April 2012; Revised 22 June 2012; Accepted 29 June 2012

Academic Editor: J. Gimble

Copyright © 2012 Ann Steele et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Human cardiac-derived progenitor cells (hCPCs) have shown promise in treating heart failure (HF) in adults. The purpose of this study was to describe derivation of hCPCs from pediatric patients with end-stage HF. Methods. At surgery, discarded right atrial tissues (hAA) were obtained from HF patients ( ; hAA-CHF). Minced tissues were suspended in complete (serum-containing) DMEM. Cells were selected for their tissue migration and expression of stem cell factor receptor (hc-kit). Characterization of cells included immunohistochemical screening with a panel of monoclonal antibodies. Results. Cells, including phase-bright cells identified as , spontaneously emigrated from hAA-CHF in suspended explant cultures (SEC) after Day 7. When cocultured with tissue, emigrated cells proliferated, first as loosely attached clones and later as multicellular clusters. At Day 21~5% of cells were . Between Days 14 and 28 cells exhibited mesodermal commitment (GATA- and ); then after Day 28 cardiac lineages ( , smooth muscle , , and myosin light ). Conclusions. hCPCs can be derived from atrial tissue of pediatric patients with end-stage HF. SEC is a novel culture method for derivation of migratory cells that favors clinical translation by reducing the need for exogenously added factors to expand hCPCs in vitro.