Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate

Figure 3

Identification of emigrated cells as c-kit^positive. (a) Cluster of c-kit^positive cells from a cytospin preparation after 28 days in culture (c-kit^positive cells (light- and dark-brown arrow) visualized using reporter chromogen DAB with nuclear hematoxylin counterstain (light blue). Magnification ×630. (b) Representative cardiosphere composed of multiple cells (arrows) that were predominantly c-kit^positive (brown to black-nickel-toned DAB and light blue nuclear hematoxylin counterstain). Magnification ×400.

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