Heart Cells with Regenerative Potential from Pediatric Patients with End Stage Heart Failure: A Translatable Method to Enrich and Propagate

Figure 4

In vitro differentiation of hc-kit^positive cells. Representative photomicrographs of emigrated and immune-selected hc-kit^positive cells demonstrating expression of mesodermal markers (GATA 4 and Nkx 2.5) and cardiac differentiation (Mcl-2 and troponin-I). Monoclonal antibody binding was visualized using DAB reporter chromogen (brown) with nuclear hematoxylin counterstain (blue). (a) GATA 4^positive hc-kit^positive cells. Magnification ×630. (b) Nkx 2.5^positive hc-kit^positive cells. Magnification ×200. (c) Mcl-2^positive hc-kit^positive cells at Day 28. Magnification ×630. (c) Troponin-I^positive hc-kit^positive cells at Day 28. Magnification ×630. Note: hc-kit^positive cells are positive for the four epitopes.

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