Clonal Populations of Amniotic Cells by Dilution and Direct Plating: Evidence for Hidden Diversity

Table 1

Independent cell populations from uncultured amniocentesis samples^a.

Sample	Dilution vol. mL	24 well plate^b	Clones^c	Doublets^d	Senescent clones^e	Total clones^f	Total viable clones^g
RC	25	1	13		1	13	12
LB	25	1	11		2	11	9
GW	100	1	3		1
		2	2
		3	3
		4	6	1		16	14
	125	1	1
		2	2
PB		3	4		1
		4	1
		5	1			10	9

^aAmniocentesis samples were donated with informed consent under approved IRB protocol.
^bDiluted samples were plated in 1 to 5 tissue-culture-treated plates. Clones were assigned unique identifier based on position and plate of derivation.
^cPopulations arising from a single spherical cluster in a single well.
^dPopulation derived from 2-well separated spherical clusters in a single well.
^ePopulations that did not continue to proliferate and were discarded after 2 weeks in culture.
^fTotal number of populations obtained from a single amniocentesis sample.
^gTotal number of viable populations expanded and cryopreserved for long-term storage.