Clonal Populations of Amniotic Cells by Dilution and Direct Plating: Evidence for Hidden Diversity

Table 3

Similarities and differences among cell populations.

	Morphology^a		Differentiation^b		Contact	Coexpressed markers stromal/	Cadherin^e
	Stromal	Epithelial	Fat	Bone	inhibition^c	epithelial^d	N	E
PB4A2	+	−	+	+	−	+	+	−
PB1C4	+	−	+	−	−	−	+	−
PB3B5	−	+	−	−	+	+	+	−
BMMSC	+	−	+	+	−	−	+	−
ChM1	+	+	ND^f	ND	ND	+	+	+

^aMorphology judged by phase microscopy of cells in newly established cultures. ChM1 mixed cell populations contained both cell types.
^bOn the basis of alizarin red and oil red-O staining of differentiated cell populations.
^cNonoverlapping epithelial cells in confluent cultures with cobblestone appearance.
^dVisible networks of keratin and vimentin in the same cell by high-resolution immunofluorescence microscopy.
^eDetected with TaqMan gene expression assays and/or by immunofluorescence analysis.
^fND: not determined.