Cellular Reprogramming Employing Recombinant Sox2 Protein
Figure 3
Reprogramming of MEFs using cell-permeant Sox2-TAT protein. (a) Schematic presentation showing the timeline of reprogramming setup. Oct4-GiP MEFs were infected with viruses encoding Oct4, Klf4, and c-Myc (OKC) at day 0. Starting at day 1 post infection (p.i.), cells were incubated with Sox2-TAT for 5 and 10 days, respectively, changing the Sox2-TAT-supplemented media daily. (b) Representative pictures of cells transduced with 200 nM Sox2-TAT protein (right panel) displaying phase contrast and GFP channel (inset) 14 days p.i. OKC-infected cells treated with medium only served as controls (left panel). Scale bar = 100 μm. (c) Quantification of GFP-positive colonies at day 16 p.i. OKC-infected cells (−) as well as SOKC-infected cells (+) served as controls.
