Generation of Human-Induced Pluripotent Stem Cells by a Nonintegrating RNA Sendai Virus Vector in Feeder-Free or Xeno-Free Conditions

Figure 1

Reprogramming workflow on feeder-free and xeno-free conditions. (1) Transduce cells with CytoTune Sendai virus vector overnight. (2) Remove virus and replace with cell’s growth medium. Cells are grown in cell’s growth medium for 6-7 days after transduction. (3) Split cells onto Geltrex-coated plates or inactivated human feeders. Culture transduced cells in feeder-free conditions with StemPro hESC SFM medium and Geltrex-coated plates or xeno-free medium consisting of KnockOut-DMEM/F-12, 15% KnockOut-SR Xeno-Free CTS, and 1X KnockOut SR Xeno-Free Growth Factor Cocktail. Colonies can be picked between 17–25 days after transduction.