Generation of Human-Induced Pluripotent Stem Cells by a Nonintegrating RNA Sendai Virus Vector in Feeder-Free or Xeno-Free Conditions

Figure 2

(A) Characterization and differentiation of iPSC colonies generated on feeder-free conditions. Colonies were stained for pluripotent markers: SSEA4 (b), SOX2 (c), Tra1-81 (e), NANOG (f), and phase ((a) and (d)). (B) These colonies were differentiated in vitro into embryoid bodies (EBs) comprising of the three embryonic germ layers: endoderm marker α fetoprotein (AFP) (g), mesodermal marker smooth muscle actin (SMA) (h), and ectoderm marker Beta 3-tubulin (Beta III) (i). (C) Colonies were stained with alkaline phosphatase staining 25 days after transduction. (D) Colonies were also subjected to directed differentiation to neural stem cells, stained with Beta 3-tubulin (Beta III) (j), Nestin (k), and random differentiation to beating cardiomyocytes (l).