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Stem Cells International
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Stem Cells International
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2012
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Article
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Tab 1
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Research Article
Feline Neural Progenitor Cells II: Use of Novel Plasmid Vector and Hybrid Promoter to Drive Expression of Glial Cell Line-Derived Neurotrophic Factor Transgene
Table 1
Primary antibodies used for immunocytochemistry on cNPCs.
Target
Antibody type
Reactivity in retina
Source
Dilutions
Nestin
Mouse monoclonal
Progenitors, reactive glia
BD
1 : 200
Vimentin
Mouse monoclonal
Progenitors, reactive glia
Sigma
1 : 200
Ki-67
Mouse monoclonal
Proliferating cells
BD
1 : 200
GFAP
Mouse monoclonal
Astrocytes, reactive glia
Chemicon
1 : 200
β
3-tubulin
Mouse monoclonal
Immature neurons
Chemicon
1 : 200
GDNF
Rabbit polyclonal
Growth factor
SCBT
1 : 200