Human Amniotic Fluid Cells Form Functional Gap Junctions with Cortical Cells

Figure 3

Cx43 expression in mouse cortical cells. (a) Immunocytochemistry showed that Cx43 (green) is expressed in Nestin- (red) positive cortical progenitors. (c) Similarly, abundant levels of Cx43 protein were detected at cell-cell boundaries in cortical astrocytes. (e) Only a limited amount of Cx43 was detected in immature neurons (arrow), whereas astrocytes maintained high degrees of Cx43 expression. (b), (d) and (f) are the corresponding phase contrast images of (a), (c) and (e), respectively. Nuclei were stained with Hoechst (blue). Scale bar: 10 μm.