Human Amniotic Fluid Cells Form Functional Gap Junctions with Cortical Cells

Figure 7

Cellular architecture in normal and injured motor cortices. (a)-(b) Hematoxylin and eosin (H&E) staining of coronal sections from mice subjected to sham surgery (a) and motor cortex injury (b). (c)-(d) Mosaic confocal images of adjacent sections show MAP2- (red) positive neurons and GFAP (green) astrocytes in sham (c) and injured (d) brains. (e)-(f) Higher magnifications of the insets in (c) and (d). In contrast to the well-organized architecture in the control motor cortex (e), there was a significant loss in the number of neurons, associated with an increase in astrogliosis in the injured cortex. Hoechst (blue) was used to label the nuclei. Scale bars: 1000 μm (a)–(d), 100 μm (e)-(f).