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Cell type | Source | Special consent required | Advantages | Disadvantages |
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Peripheral blood mononuclear cells (PBMCs) | By product of leukoreduction | No | Donor with known phenotype | Limited expansion capacity |
Apheresis collection | Yes | ≥3 products from single collection | Donors may become ineligible |
Mobilizing agent before apheresis | Yes (for mobilization and apheresis) | Expansion similar to cord blood with humanized media | Quality control requirement greater than blood |
Cord blood | Low volume units unsuitable for transplantation | Yes | Hematopoietic progenitor cell number > PBMC 3–50 products phenotype must be determined | Multiple donations not possible |
Quality control moderately complex |
iPSC | Fibroblast cultures from autologous or allogenic donors of known phenotype | Yes (for skin biopsy, fibroblast culture, and induction of pluripotency) | Unlimited expansion capacity | Quality control highly complex |
hESC | Human embryos ≃1200 cell lines available | Yes | Unlimited expansion capacity | Ethical limitations on development and use |
Quality control highly complex |
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