Research Article

Comprehensive Characterization of Mesenchymal Stem Cells from Human Placenta and Fetal Membrane and Their Response to Osteoactivin Stimulation

Figure 2

The Cell analysis strategy allows the isolation of 4 different cell populations. Cells were stained with mouse anti human CD45-Amcyan, CD34-FITC, CD29-APC-CY7, CD90-AF700, CD73-PE, CD105-AF647. After FSC-A/SSC-A selection, only CD45 and CD34 cells were considered. CD73, CD105, CD90, and CD29 profiles were then analyzed. CD73+ CD105+ cells were more than 85% to 98% positive for CD90 and CD29 (lower panel). However, CD90+ CD29+ represented a more heterogenous population when looking at CD73, CD105 stainings. We defined different cell populations based on CD90 and CD29 subpopulation: CD90+ CD29+; CD90 CD29+; CD90+ CD29; CD90 CD29 (middle panel).
658356.fig.002