Comprehensive Characterization of Mesenchymal Stem Cells from Human Placenta and Fetal Membrane and Their Response to Osteoactivin Stimulation

Figure 4

PCA representation of transcriptomic comparison of Pl-MSCs, Mb-MSCs and BM-MSCs and ingenuity pathways analysis. (a) Mb-MSCs (red) and Pl-MSCs (green) are overlapping clearly indicating the impossibility to differentiate these MSCs at a transcriptional level. They can clearly be differentiated from bone marrow MSCs (blue). (b) The PCA overlapping between every cell sorted sub-population from membrane (CD29⁺ CD90⁺ red; CD29⁻ CD90⁺ blue; CD90⁻ CD29⁺ green) or nonsorted cells (Mb-MSCs purple) indicate the impossibility to discriminate the different MSCs subpopulation at a transcriptional level. (c) PCA of different MSC subpopulation from placenta (CD29⁺ CD90⁺ red; CD29⁻ CD90⁺ blue) or directly isolated cells (Pl-MSCs blue) indicate the impossibility to discriminate these MSCs subpopulation at a transcriptional level. (d) Ingenuity pathway analysis was able to define enriched pathways implicated in embryonic morphogenesis and organ development in fetal MSCs compared to BM-MSCs. (e) Ingenuity pathway analysis using organ formation and osteoblast differentiation molecular network shows an enrichment in proosteogenic genes in fetal MSCs compared to BM-MSCs.