Characterization of Porcine Ventral Mesencephalic Precursor Cells following Long-Term Propagation in 3D Culture

Figure 4

Expression of stem cell markers and markers of glial and neuronal cells in primary (noncultured) porcine ventral mesencephalon (VM) obtained at embryonic day 28–30. Photomicrographs showing an intact VM (a), and immunoexpression of the proliferation marker Ki67 (b), the proneural markers Pax6 (c) and MASH1 (d), the neural stem cell marker nestin (e), the glial markers vimentin (f) and GFAP (g), the oligodendrocyte marker CNPase (h), the neuronal markers β Tubulin III (i), MAP2 (j) and NeuN (k), and the dopaminergic marker TH (l). Scale bars: 100 μm (overview) and 20 μm (insert).

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