Nonhuman Primate Induced Pluripotent Stem Cells in Regenerative Medicine
Figure 4
Derivation of neural progenitor cells (NPCs) from marmoset iPS cells and differentiation of NPCs to mature neurons. The series (a)–(c) shows the transition from undifferentiated iPS cells (a), to a line of NPCs (b), to mature neurons (c) (100x phase-contrast images). NPCs placed on a polylysine/laminin-coated glass surface stop dividing and form extensive axons and dendrites. Details of this further maturation are shown in series (d)–(f) (400x differential interference contrast images). Note particularly the varicosities of different sizes indicated by arrows in (f). These are sites of accumulation of cellular organelles and are precursors to the formation of synapses [37]. Their presence indicates the degree of maturity of these neurons.
