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Figure 3: (a) Discarded burn skin. (b–d) Immunohistochemical images of section from discarded skin biopsies: (b) stained with PDGFRβ (Alexa fluor 488), (c) Hoechst for nuclei, and (d) overlay of PDGFRβ and Hoechst. (e) Stem cell isolation process from the adipose tissue layer of discarded burn skin (dsASCs) using the Transpose RTtissue processing system. (f) Fluorescent-activated cell sorting (FACS) analysis of P1 dsASCs stained with phycoerythrin-labeled PDGFRβ. (g–i) Immunocytochemical images of P1 dsASCs: (g) stained with PDGFRβ (Alexafluor 594), (h) Hoechst for nuclei, and (i) overlay of PDGFRβ and Hoechst. Original magnification: ×200 (b–d and dsASCs in (e)) and ×600 (g–i).