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Stem Cells International
Volume 2012, Article ID 915160, 7 pages
http://dx.doi.org/10.1155/2012/915160
Research Article

Growth Factors Released from Gelatin Hydrogel Microspheres Increase New Neurons in the Adult Mouse Brain

1Department of Developmental and Regenerative Biology, Nagoya City University Graduate School of Medical Sciences, Aichi 467-8601, Nagoya, Japan
2Department of Neonatology and Pediatrics, Nagoya City University Graduate School of Medical Sciences, Aichi 467-8601, Nagoya, Japan
3Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan

Received 23 June 2012; Accepted 9 August 2012

Academic Editor: Oscar Gonzalez-Perez

Copyright © 2012 Kanako Nakaguchi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Recent studies have shown that new neurons are continuously generated by endogenous neural stem cells in the subventricular zone (SVZ) of the adult mammalian brain. Some of these new neurons migrate to injured brain tissues and differentiate into mature neurons, suggesting that such new neurons may be able to replace neurons lost to degenerative disease or injury and improve or repair neurological deficits. Here, we tested whether delivering growth factors via gelatin hydrogel microspheres would support neurogenesis in the SVZ. Insulin-like growth factor-1 (IGF-1)-containing microspheres increased the number of new neurons in the SVZ. Hepatocyte growth factor (HGF)-containing microspheres increased the number of new neurons migrating from the SVZ towards the injured striatum in a stroke model in mouse. These results suggest that the strategy of using gelatin hydrogel microspheres to achieve the sustained release of growth factors holds promise for the clinical regeneration of damaged brain tissues from endogenous neural stem cells in the adult SVZ.