Laser-Based Propagation of Human iPS and ES Cells Generates Reproducible Cultures with Enhanced Differentiation Potential
Figure 3
Quality of iPSC cultures after laser-mediated passage. (a) Immunocytochemical analysis of Oct4, Sox2, Nanog, SSEA4, TRA1-60, and TRA1-81 expression immediately following laser-mediated sectioning of iPSC cultures (BIMR L) into 200 μm sections. Hoechst was used as a nuclear counterstain. Note that all markers are expressed homogeneously across iPSC clumps. Scale bar, 1 mm. (b) Immunocytochemical analysis of apoptosis markers, caspase-3, and cleaved PARP, following laser-mediated sectioning of iPSC cultures. Hoechst was used as a nuclear counterstain. Scale bar, 1 mm. (c) Analysis of iPSC (BIMR 6, left) and ESC (H9, right) growth following propagation using laser-mediated passage or collagenase passage. indicates passage number. Data are shown as mean + s.d. ().
