Table of Contents Author Guidelines Submit a Manuscript
Stem Cells International
Volume 2013, Article ID 245695, 9 pages
http://dx.doi.org/10.1155/2013/245695
Research Article

Proteomic Profiling of Ex Vivo Expanded CD34-Positive Haematopoetic Cells Derived from Umbilical Cord Blood

1Molecular Proteomics Laboratory (MPL), Center for Biomedical Research (BMFZ), Heinrich Heine University, Universitätsstrasse 1, 40225 Düsseldorf, Germany
2Institute for Transplantation Diagnostics and Cell Therapeutics, Heinrich Heine University Medical Center, 40225 Düsseldorf, Germany

Received 12 October 2012; Revised 25 January 2013; Accepted 7 February 2013

Academic Editor: David Allan

Copyright © 2013 Heiner Falkenberg et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. S. S. Kelly, C. B. S. Sola, M. De Lima, and E. Shpall, “Ex vivo expansion of cord blood,” Bone Marrow Transplantation, vol. 44, no. 10, pp. 673–681, 2009. View at Publisher · View at Google Scholar · View at Scopus
  2. J. E. Wagner and E. Gluckman, “Umbilical cord blood transplantation: the first 20 years,” Seminars in Hematology, vol. 47, no. 1, pp. 3–12, 2010. View at Publisher · View at Google Scholar · View at Scopus
  3. M. N. Fernández, “Improving the outcome of cord blood transplantation: use of mobilized HSC and other cells from third party donors,” British Journal of Haematology, vol. 147, no. 2, pp. 161–176, 2009. View at Publisher · View at Google Scholar · View at Scopus
  4. E. Csaszar, D. C. Kirouac, M. Yu, W. Wang et al., “Rapid expansion of human hematopoietic stem cells by automated control of inhibitory feedback signaling,” Cell Stem Cell, vol. 10, no. 2, pp. 218–229, 2012. View at Publisher · View at Google Scholar
  5. C. Delaney, S. Heimfeld, C. Brashem-Stein, H. Voorhies, R. L. Manger, and I. D. Bernstein, “Notch-mediated expansion of human cord blood progenitor cells capable of rapid myeloid reconstitution,” Nature Medicine, vol. 16, no. 2, pp. 232–236, 2010. View at Publisher · View at Google Scholar · View at Scopus
  6. M. Bantscheff, S. Lemeer, M. M. Savitski, and B. Kuster, “Quantitative mass spectrometry in proteomics: critical review update from 2007 to the present,” Analytical and Bioanalytical Chemistry, vol. 404, no. 4, pp. 939–965, 2012. View at Publisher · View at Google Scholar
  7. C. Zenzmaier, M. Kollroser, B. Gesslbauer, A. Jandrositz, K. H. Preisegger, and A. J. Kungl, “Preliminary 2-D chromatographic investigation of the human stem cell proteome,” Biochemical and Biophysical Research Communications, vol. 310, no. 2, pp. 483–490, 2003. View at Publisher · View at Google Scholar · View at Scopus
  8. C. Zenzmaier, B. Gesslbauer, N. Grobuschek, A. Jandrositz, K. H. Preisegger, and A. J. Kungl, “Proteomic profiling of human stem cells derived from umbilical cord blood,” Biochemical and Biophysical Research Communications, vol. 328, no. 4, pp. 968–972, 2005. View at Publisher · View at Google Scholar · View at Scopus
  9. W. Tao, M. Wang, E. D. Voss et al., “Comparative proteomic analysis of human CD34+ stem/progenitor cells and mature CD15+ myeloid cells,” Stem Cells, vol. 22, no. 6, pp. 1003–1014, 2004. View at Google Scholar · View at Scopus
  10. F. Liu, J. Lu, H. H. Fan et al., “Insights into human CD34+ hematopoietic stem/progenitor cells through a systematically proteomic survey coupled with transcriptome,” Proteomics, vol. 6, no. 9, pp. 2673–2692, 2006. View at Publisher · View at Google Scholar · View at Scopus
  11. A. D'Alessandro, G. Liumbruno, G. Grazzini, S. Pupella, L. Lombardini, and L. Zolla, “Umbilical cord blood stem cells: towards a proteomic approach,” Journal of Proteomics, vol. 73, no. 3, pp. 468–482, 2010. View at Publisher · View at Google Scholar · View at Scopus
  12. A. D'Alessandro, G. Grazzini, B. Giardina, and L. Zolla, “In silico analyses of proteomic data suggest a role for heat shock proteins in umbilical cord blood hematopoietic stem cells,” Stem Cell Reviews and Reports, vol. 6, no. 4, pp. 532–547, 2010. View at Publisher · View at Google Scholar · View at Scopus
  13. K. A. Neilson, N. A. Ali, S. Muralidharan et al., “Less label, more free: approaches in label-free quantitative mass spectrometry,” Proteomics, vol. 11, no. 4, pp. 535–553, 2011. View at Publisher · View at Google Scholar
  14. M. C. Wiener, J. R. Sachs, E. G. Deyanova, and N. A. Yates, “Differential mass spectrometry: a label-free LC-MS method for finding significant differences in complex peptide and protein mixtures,” Analytical Chemistry, vol. 76, no. 20, pp. 6085–6096, 2004. View at Publisher · View at Google Scholar · View at Scopus
  15. B. Sitek, D. M. Waldera-Lupa, G. Poschmann, H. E. Meyer, and K. Stühler, “Application of label-free proteomics for differential analysis of lung carcinoma cell line A549,” Methods in Molecular Biology, vol. 893, pp. 241–248, 2012. View at Publisher · View at Google Scholar
  16. G. Kögler, J. Callejas, P. Hakenberg et al., “Hematopoietic transplant potential of unrelated cord blood: critical issues,” Journal of Hematotherapy, vol. 5, no. 2, pp. 105–116, 1996. View at Publisher · View at Google Scholar
  17. G. Kögler, T. F. Radke, A. Lefort et al., “Cytokine production and hematopoiesis supporting activity of cord blood-derived unrestricted somatic stem cells,” Experimental Hematology, vol. 33, no. 5, pp. 573–583, 2005. View at Publisher · View at Google Scholar · View at Scopus
  18. M. V. Nesterenko, M. Tilley, and S. J. Upton, “A simple modification of Blum's silver stain method allows for 30 minute detection of proteins in polyacrylamide gels,” Journal of Biochemical and Biophysical Methods, vol. 28, no. 3, pp. 239–242, 1994. View at Publisher · View at Google Scholar · View at Scopus
  19. J. Cox and M. Mann, “MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification,” Nature Biotechnology, vol. 26, no. 12, pp. 1367–1372, 2008. View at Publisher · View at Google Scholar · View at Scopus
  20. C. A. Luber, J. Cox, H. Lauterbach et al., “Quantitative proteomics reveals subset-specific viral recognition in dendritic cells,” Immunity, vol. 32, no. 2, pp. 279–289, 2010. View at Publisher · View at Google Scholar · View at Scopus
  21. L. J. Jensen, M. Kuhn, M. Stark et al., “STRING 8—a global view on proteins and their functional interactions in 630 organisms,” Nucleic Acids Research, vol. 37, no. 1, pp. D412–D416, 2009. View at Publisher · View at Google Scholar · View at Scopus