Comparative Evaluation of Human Mesenchymal Stem Cells of Fetal (Wharton's Jelly) and Adult (Adipose Tissue) Origin during Prolonged In Vitro Expansion: Considerations for Cytotherapy

Figure 4

Immunophenotypic analysis of WJSC and ADSC for the expression of typical mesenchymal and hemopoietic stem cell surface markers (first five and last three markers, resp.) during subculture. The vertical axes of the histograms depict event counts, and the horizontal axes represent fluorescence intensities of surface-bound conjugates, that is, marker expression levels. Staining of WJSC and ADSC with specific monoclonal antibody conjugates is illustrated by solid dark histograms (MSC markers: CD-29 and -44 FITC; CD-73, -90, and -105 PE) and by open histograms (HSC markers: CD-14 and -34 ECD; CD-45 PC5). Stripped histograms correspond to isotype-matched (negative) controls.