Comparative Evaluation of Human Mesenchymal Stem Cells of Fetal (Wharton's Jelly) and Adult (Adipose Tissue) Origin during Prolonged In Vitro Expansion: Considerations for Cytotherapy
Table 1
Cell isolation and culture establishment efficiency.
WJSC
ADSC
value
Mean tissue length-weight
13.3 cm ± 2*
173.8 g ± 135.7
—
Mean nucleated cell yield (×106) (per unit length-weight)
2.28 ± 1.55 (0.17/cm)
312 ± 198 (1.8/g)
—
Mean viability at isolation (%)
94.3 ± 2.2
96.6 ± 1.6
>0.05
Mean viability at resuscitation (%)
92.9 ± 4.3
92.7 ± 4.9
>0.05
% of adherent cells in starting culture
17.3 ± 4.6
0.93 ± 0.45
<0.001
Adherent cultures beyond p.3
100% (5/5)
37.5% (3/8)
<0.05
Mean passage-to-passage expansion rate % (p.1–5)
974 ± 317
175 ± 56
<0.01
Mean subculture viability % (p.1–5)
97.6 ± 0.5
96.4 ± 1.9
>0.05
value, WJSC versus ADSC. *A statistically significant association was found between UC tissue sample length and the yield of isolated nucleated cells following tissue processing (see text for details).
