Age-Related Yield of Adipose-Derived Stem Cells Bearing the Low-Affinity Nerve Growth Factor Receptor

Table 1

Primer sets and annealing temperatures used for reverse transcription PCR.

Gene	Primer sequence (5′-3′)	Annealing temperature
Sox2
(fwd); (rev)	ACTTTTGTCGGAGACGGAGA GTTCATGTGCGCGTAACTGT	55°C
Notch1
(fwd); (rev)	TCACGCTGACGGAGTACAAG CCACACTCGTTGACATCCTG	55°C
Rex1
(fwd); (rev)	GGCGGAAATAGAACCTGTCA CTTCCAGGATGGGTTGAGAA	55°C
Oct4
(fwd); (rev)	AGTGAGAGGCAACCTGGAGA ACACTCGGACCACATCCTTC	55°C
Nanog
(fwd); (rev)	TTCCTTCCTCCATGGATCTG TCTGCTGGAGGCTGAGGTAT	55°C
Nestin
(fwd); (rev)	AACAGCGACGGAGGTCTCTA TTCTCTTGTCCCGCAGACTT	60°C
GAPDH
(fwd); (rev)	GAGTCAACGGATTTGGTCGT TTGATTTTGGAGGGATCTCG	60°C

Sox2: SRY (sex determining region Y)-box 2; Notch1: Notch homolog 1; Oct4: octamer-binding transcription factor 4; GAPDH: glyceraldehyde 3-phosphate dehydrogenase.