Different Culture Media Affect Proliferation, Surface Epitope Expression, and Differentiation of Ovine MSC

Table 3

Adipogenic differentiation media according to standard protocols.

Adipogenic differentiation	Protocol 1 [31]	Protocol 2 [21]
Adipogenic induction medium
DMEM 4.5 g/L glucose
FCS [%]	10	10
Dexamethasone [µM]	1	1
Indomethacin [µM]	0.2	200
Insulin [mg/mL]	0.01	0.01
3-Isobutylxanthin [mM]	0.05	0.5
Cell density [/cm^2]	80.000	30.000
Adipogenic maintenance medium
Serum [%]	10
Insulin [mg/mL]	0.01