Different Culture Media Affect Proliferation, Surface Epitope Expression, and Differentiation of Ovine MSC

Table 5

Antibodies used for flow cytometry analysis of oMSC to detect surface epitopes.

Antibody	Alternate name	Reactivity	Identical amino acid residues between man and sheep [%]^3	Volume [µL] per test (in 100 µL PBS)
CD29^1	Integrin beta 1	PE rabbit anti-human	93	10
CD44^2	H-CAM, PGP-1	FITC mouse anti-sheep	—	5
CD45^1	Leukocyte common antigen	FITC mouse anti-sheep	—	10
CD73^1	NT5E, 5′ nucleotidase, E5NT, eNT, NT, NT5	APC mouse anti-human	81–89	3
CD90^1	Thy-1	FITC mouse anti-human	—	3
CD105^1	Endoglin, ENG, END, HHT1, ORW, ORW1	PE mouse anti-human	70–75	3
CD166^1	ALCAM	PE mouse anti-human	90	10

1BD Biosciences, Beckton Dickinson, Franklin Lakes, USA.
²Serotec, Oxford, England.
³Protein BLAST sequence alignment, ncbi database (including transcript and splicing variants).