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Stem Cells International
Volume 2013 (2013), Article ID 659739, 11 pages
http://dx.doi.org/10.1155/2013/659739
Research Article

Distinct iPS Cells Show Different Cardiac Differentiation Efficiency

1Department of Cardiology, Keio University School of Medicine, Tokyo 160-8582, Japan
2Biomedical Research Laboratories, Asubio Pharma Co., Ltd., Kobe 650-0047, Japan

Received 5 June 2013; Revised 18 August 2013; Accepted 3 September 2013

Academic Editor: Ildiko Bock-Marquette

Copyright © 2013 Yohei Ohno et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Patient-specific induced pluripotent stem (iPS) cells can be generated by introducing transcription factors that are highly expressed in embryonic stem (ES) cells into somatic cells. This opens up new possibilities for cell transplantation-based regenerative medicine by overcoming the ethical issues and immunological problems associated with ES cells. Despite the development of various methods for the generation of iPS cells that have resulted in increased efficiency, safety, and general versatility, it remains unknown which types of iPS cells are suitable for clinical use. Therefore, the aims of the present study were to assess (1) the differentiation potential, time course, and efficiency of different types of iPS cell lines to differentiate into cardiomyocytes in vitro and (2) the properties of the iPS cell-derived cardiomyocytes. We found that high-quality iPS cells exhibited better cardiomyocyte differentiation in terms of the time course and efficiency of differentiation than low-quality iPS cells, which hardly ever differentiated into cardiomyocytes. Because of the different properties of the various iPS cell lines such as cardiac differentiation efficiency and potential safety hazards, newly established iPS cell lines must be characterized prior to their use in cardiac regenerative medicine.