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Figure 2: Cardiomyocyte differentiation efficiency of pluripotent stem cells and temporal gene expression patterns during cardiomyocyte differentiation. (a) Percentage of beating colonies on days 6–15 in mouse embryonic stem (ES) cells, Nanog induced pluripotent stem (iPS) cells, and Fbx15-iPS cells. Data are the mean SEM ( in all groups). ((b)–(g)) Quantitative RT-PCR analyses showing temporal gene expression patterns of the mesodermal marker Brachyury (b), the early cardiac mesodermal marker Mesp1 (c), the cardiac-specific transcription factors Nkx2.5 (d) and Gata4 (e), and the cardiac-specific proteins Nppa (f) and Myl2 (g) in EB3 ES cells (closed squares), 20D17 Nanog-iPS cells (open circles), and WT-1 Fbx15-iPS cells (open triangles). Data are the mean SEM ( in all groups).