Research Article

Distinct iPS Cells Show Different Cardiac Differentiation Efficiency

Figure 3

Transgene expression profiles of purified cardiomyocytes derived from Nanog and Fbx15 induced pluripotent stem (iPS) cells. Total RNA was isolated from purified cardiomyocytes derived from mouse embryonic stem (ES) cells, three Nanog-iPS cell clones (20D17, 38C2, and 38D2), two Fbx15-iPS cell clones (WT-1 and 4-3), undifferentiated Fbx15-iPS cells, and the mouse heart. RT-PCR analyses were performed to determine the transgene expression of the four transcription factors (Oct3/4, Sox2, Klf4, and c-Myc), with GAPDH used as an internal control.
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