Developing a New Two-Step Protocol to Generate Functional Hepatocytes from Wharton’s Jelly-Derived Mesenchymal Stem Cells under Hypoxic Condition
Figure 5
The capacities of the differentiated cells to store glycogen and accumulate LDL inside the cells after induction for 18 days. Glycogen storage of MSCDHCs generated from WJMSCs-SUT1 ((i): (A), (C), (E), and (G)) and WJMSCs-SUT2 ((i): (B), (D), (F), and (H)) were characterized by PAS staining following the differentiation period. LDL-uptake capacity of MSCDHCs generated from WJMSCs-SUT1 ((ii): (a), (c), (e), and (g)) and WJMSCs-SUT2 ((ii): (b), (d), (f), and (h)) were analyzed by LDL-uptake assay following the differentiation period. Original magnifications: for all pictures. Bars indicate 100 μm.
