Research Article
Developing a New Two-Step Protocol to Generate Functional Hepatocytes from Wharton’s Jelly-Derived Mesenchymal Stem Cells under Hypoxic Condition
Table 1
List of primer sequences used in this study.
| Gene | Primer sequence (5′ to 3′) | Annealing Temp. | Size (bp) | Reference |
| AFP | GCTTGGTGGTGGATGAAACA TCCTCTGTTATTTGTGGCTTTTG | 62 | 157 | [22] | ALB | TGAGAAAACGCCAGTAAGTGAC TGCGAAATCATCCATAACAGC | 62 | 265 | [22] | CYP3A4 | CCTTACAT TACACACCCTTTGGAAGT AGCTCAATGCATGTACAGAATCCCCGGTTA | 62 | 382 | [22] | HNF4α | GCCTACCTCAAAGCCATCAT GACCCTCCCAGCAGCATCTC | 62 | 275 | [22] | AAT | ACATTTACCCAAACTGTCCATT GCTTCAGTCCCTTTCTCGTC | 56 | 183 | [23] | TTR | GGTGAATCCAAGTGTCCTCTGAT GTGACGACAGCCGTGGTGGAA | 61 | 352 | [24] | G6P | GCTGGAGTCCTGTCAGGCATTGC TAGAGCTGAGGCGGAATGGGAG | 56 | 350 | [23] | TAT | CCCCTGTGGGTCAGTGTT GTGCGACATAGGATGCTTTT | 56 | 345 | [23] | GAPDH | AGCCACATCGCTCAGACACC GTACTCAGCGGCCAGCATCG | 60 | 302 | [25] |
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ALB: albumin; AFP: alpha-fetoprotein; AAT: alpha-1-antitrypsin; CYP3A4: cytochrome P450 type 3A4; GAPDH: glyceraldehyde-3-phosphate dehydrogenase; G6P: glucose-6-phosphatase; HNF4α: hepatocyte nuclear factor 4α; TAT: tyrosine aminotransferase; TTR: transthyretin; Temp.: temperature; bp: base pair.
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