Microarray-Based Comparisons of Ion Channel Expression Patterns: Human Keratinocytes to Reprogrammed hiPSCs to Differentiated Neuronal and Cardiac Progeny

Table 1

The expression levels for ion channel genes were compared in hiPSCs (9 samples) and keratinocytes (6 samples) via microarray. Gene names, accession numbers, and gene symbols are shown together with the corresponding fold change and 𝑃 values. Significant changes (defined as fold change > 2, 𝑃<0.05) are labeled in bold for upregulation or italic for downregulation. Accession numbers refer to the NCBI or Ensembl (numbers starting with ENST) databases. Analyses of hiPSC-derived neurons or hiPSC-derived cardiomyocytes were performed using published data sets from GSE34879 (neurons) and GSE17579 (cardiomyocytes). These data sets already contain both iPSCs and differentiated progeny. Fold changes > 2 (marked bold) and <−2 (marked italic) are shown in the respective columns.

(a) Voltage-gated calcium channels

Gene name	Accession number	Gene symbol	𝑃	Fold change; Ker → hiPSC	Fold change; hiPSC → Neuron	Fold change hiPSC → CM
Calcium channel, voltage-dependent, P/Q type, alpha 1A subunit, transcript variant 1	NM_000068	CACNA1A	2.26𝐸−01	−1.32
Calcium channel, voltage-dependent, N type, alpha 1B subunit	NM_000718	CACNA1B	7.27𝐸−01	−1.10
Calcium channel, voltage-dependent, N type, alpha 1B subunit [Source: HGNC Symbol; Acc: 1389]	ENST00000277550	CACNA1B	7.15𝐸−01	−1.14
Calcium channel, voltage-dependent, N type, alpha 1B subunit	NM_000718	CACNA1B	𝟔.𝟑𝟐𝐄−𝟎𝟗	6.82
Calcium channel, voltage-dependent, N type, alpha 1B subunit [Source: HGNC Symbol; Acc: 1389]	ENST00000277551	CACNA1B	3.32𝐸−02	−1.57
Calcium channel, voltage-dependent, L type, alpha 1C subunit, transcript variant 18	NM_000719	CACNA1C	6.77𝐸−02	−1.37
Calcium channel, voltage-dependent, L type, alpha 1C subunit, transcript variant 18	NM_000719	CACNA1C	𝟓.𝟕𝟐𝐄−𝟎𝟑	2.37		2.94
Calcium channel, voltage-dependent, L type, alpha 1D subunit, transcript variant 1	NM_000720	CACNA1D	3.83𝐸−01	1.58
Calcium channel, voltage-dependent, R type, alpha 1E subunit, transcript variant 3	NM_000721	CACNA1E	3.10 𝐸−02	−4.48	2.08
Calcium channel, voltage-dependent, R type, alpha 1E subunit [Source: HGNC Symbol; Acc: 1392]	ENST00000524607	CACNA1E	8.83𝐸−01	1.05
Calcium channel, voltage-dependent, L type, alpha 1F subunit	NM_005183	CACNA1F	8.92𝐸−01	1.02
Calcium channel, voltage-dependent, T type, alpha 1G subunit, transcript variant 15	NM_198397	CACNA1G	6.42𝐸−01	−1.12	4.60
Calcium channel, voltage-dependent, T type, alpha 1G subunit, transcript variant 1	NM_018896	CACNA1G	𝟓.𝟑𝟕𝐄−𝟎𝟕	6.96
Calcium channel, voltage-dependent, T type, alpha 1H subunit, transcript variant 1	NM_021098	CACNA1H	𝟕.𝟑𝟖𝐄−𝟏𝟎	6.78
Calcium channel, voltage-dependent, T type, alpha 1I subunit, transcript variant 1	NM_021096	CACNA1I	𝟐.𝟐𝟐𝐄−𝟏𝟏	37.25
Calcium channel, voltage-dependent, T type, alpha 1I subunit, transcript variant 1	NM_021096	CACNA1I	7.56𝐸−02	−1.56
Calcium channel, voltage-dependent, L type, alpha 1S subunit	NM_000069	CACNA1S	5.22𝐸−02	−1.97
Calcium channel, voltage-dependent, alpha 2/delta subunit 1	NM_000722	CACNA2D1	𝟏.𝟑𝟖𝐄−𝟎𝟗	9.65
Calcium channel, voltage-dependent, alpha 2/delta subunit 2, transcript variant 1	NM_001005505	CACNA2D2	𝟑.𝟑𝟎𝐄−𝟏𝟎	62.50	2.02
Calcium channel, voltage-dependent, alpha 2/delta subunit 3	NM_018398	CACNA2D3	𝟓.𝟏𝟒𝐄−𝟎𝟓	5.42	2.55
Calcium channel, voltage-dependent, alpha 2/delta subunit 3	AF516696	CACNA2D3	𝟒.𝟔𝟖𝐄−𝟏𝟏	33.67
Calcium channel, voltage-dependent, alpha 2/delta subunit 4	NM_172364	CACNA2D4	2.95𝐸−01	−1.41
Calcium channel, voltage-dependent, beta 1 subunit, transcript variant 3	NM_199248	CACNB1	4.31𝐸−01	−1.14
Calcium channel, voltage-dependent, beta 1 subunit, transcript variant 1	NM_000723	CACNB1	8.02𝐸−01	−1.02
Calcium channel, voltage-dependent, beta 1 subunit, transcript variant 1	NM_000723	CACNB1	6.22𝐸−01	1.04
cDNA FLJ45229 fis, clone BRCAN2020972,	AK128769	CACNB2	9.78𝐸−01	1.02
Calcium channel, voltage-dependent, beta 2 subunit, transcript variant 1	NM_000724	CACNB2	𝟒.𝟕𝟏𝐄−𝟎𝟐	3.45
Calcium channel, voltage-dependent, beta 3 subunit, transcript variant 1	NM_000725	CACNB3	3.55𝐸−02	−1.93	9.56
Calcium channel, voltage-dependent, beta 3 subunit, transcript variant 1	NM_000725	CACNB3	6.61𝐸−01	1.09
Calcium channel, voltage-dependent, beta 4 subunit, transcript variant 1	NM_001005747	CACNB4	𝟏.𝟑𝟑𝐄−𝟎𝟑	4.43
Calcium channel, voltage-dependent, gamma subunit 1	NM_000727	CACNG1	9.46𝐸−01	1.02
Calcium channel, voltage-dependent, gamma subunit 2	NM_006078	CACNG2	4.19𝐸−01	−1.29
Calcium channel, voltage-dependent, gamma subunit 2	NM_006078	CACNG2	7.91𝐸−01	1.09
Calcium channel, voltage-dependent, gamma subunit 3	NM_006539	CACNG3	2.21𝐸−01	−1.95
Calcium channel, voltage-dependent, gamma subunit 4	NM_014405	CACNG4	4.93𝐸−01	1.14
Calcium channel, voltage-dependent, gamma subunit 5	NM_145811	CACNG5	7.49𝐸−01	−1.13
Calcium channel, voltage-dependent, gamma subunit 5 [Source: HGNC Symbol; Acc: 1409]	ENST00000169565	CACNG5	2.19𝐸−01	−1.56
Calcium channel, voltage-dependent, gamma subunit 6, transcript variant 1	NM_145814	CACNG6	𝟓.𝟓𝟔𝐄−𝟏𝟏	46.40
Calcium channel, voltage-dependent, gamma subunit 7	NM_031896	CACNG7	𝟑.𝟓𝟒𝐄−𝟏𝟑	1439.36	3.37
Calcium channel, voltage-dependent, gamma subunit 7	NM_031896	CACNG7	1.82𝐸−01	1.26
Calcium channel, voltage-dependent, gamma subunit 8	NM_031895	CACNG8	2.01𝐸−01	1.43
Calcium channel, voltage-dependent, gamma subunit 8	NM_031895	CACNG8	𝟏.𝟏𝟖𝐄−𝟎𝟗	9.70

(b) Sperm-associated cation channels

Gene name	Accession number	Gene symbol	𝑃	Fold change; Ker → hiPSC	Fold change; hiPSC → Neuron	Fold change hiPSC → CM
Cation channel, sperm associated 1	NM_053054	CATSPER1	3.26𝐸−01	1.51
Cation channel, sperm associated 2, transcript variant 2	NM_172095	CATSPER2	1.32 𝐸−04	−2.78
Cation channel, sperm associated 2, transcript variant 4	NM_172097	CATSPER2	3.12𝐸−02	−1.78
Cation channel, sperm associated 3	NM_178019	CATSPER3	1.50𝐸−03	1.93
Cation channel, sperm associated 4	NM_198137	CATSPER4	2.51𝐸−01	1.48
Cation channel, sperm-associated, beta	NM_024764	CATSPERB	4.38𝐸−01	−1.25
Cation channel, sperm-associated, gamma	NM_021185	CATSPERG	3.56𝐸−01	1.34

(c) Nicotinic acetylcholine receptors

Gene name	Accession number	Gene symbol	𝑃	Fold change; Ker → hiPSC	Fold change; hiPSC → Neuron	Fold change hiPSC → CM
Cholinergic receptor, nicotinic, alpha 1 (muscle), transcript variant 1	NM_001039523	CHRNA1	8.06𝐸−01	−1.10
Cholinergic receptor, nicotinic, alpha 1 (muscle), (cDNA clone IMAGE: 4124038), with apparent retained intron	BC006314	CHRNA1	4.64𝐸−01	1.47
Cholinergic receptor, nicotinic, alpha 2 (neuronal)	NM_000742	CHRNA2	6.05𝐸−01	−1.30
Cholinergic receptor, nicotinic, alpha 3, transcript variant 1	NM_000743	CHRNA3	𝟏.𝟖𝟒𝐄−𝟏𝟎	34.22
Cholinergic receptor, nicotinic, alpha 4	NM_000744	CHRNA4	6.79𝐸−01	1.15
Cholinergic receptor, nicotinic, alpha 4 (neuronal), exon 1	X89741	CHRNA4	2.79 𝐸−02	−2.53
Cholinergic receptor, nicotinic, alpha 5	NM_000745	CHRNA5	𝟒.𝟎𝟑𝐄−𝟎𝟓	3.41
Cholinergic receptor, nicotinic, alpha 6, transcript variant 1	NM_004198	CHRNA6	2.23𝐸−01	−1.27	2.21
Cholinergic receptor, nicotinic, alpha 7, transcript variant 1	NM_000746	CHRNA7	4.61𝐸−04	1.86
Cholinergic receptor, nicotinic, alpha 7, transcript variant 2	NM_001190455	CHRNA7	1.56𝐸−11	13.24
Cholinergic receptor, nicotinic, alpha 9	NM_017581	CHRNA9	𝟔.𝟑𝟎𝐄−𝟎𝟔	6.15
Cholinergic receptor, nicotinic, alpha 10	NM_020402	CHRNA10	1.57𝐸−02	1.57
Cholinergic receptor, nicotinic, beta 1 (muscle)	NM_000747	CHRNB1	8.07 𝐸−04	−2.47
Cholinergic receptor, nicotinic, beta 1 (muscle)	NM_000747	CHRNB1	8.87𝐸−03	−1.92
Cholinergic receptor, nicotinic, beta 2 (neuronal)	NM_000748	CHRNB2	6.03 𝐸−04	−3.01
Cholinergic receptor, nicotinic, beta 2 (neuronal) [Source: HGNC Symbol; Acc: 1962]	ENST00000368476	CHRNB2	𝟐.𝟗𝟖𝐄−𝟎𝟗	32.05
Cholinergic receptor, nicotinic, beta 3	NM_000749	CHRNB3	5.91𝐸−01	1.12
Cholinergic receptor, nicotinic, beta 4	NM_000750	CHRNB4	𝟏.𝟕𝟔𝐄−𝟎𝟐	4.20
Cholinergic receptor, nicotinic, delta	NM_000751	CHRND	9.84𝐸−01	1.01
Cholinergic receptor, nicotinic, epsilon	NM_000080	CHRNE	3.66𝐸−01	−1.20
Cholinergic receptor, nicotinic, gamma	NM_005199	CHRNG	6.70𝐸−01	1.14

(d) Cyclic nucleotide-gated channels

Gene name	Accession number	Gene symbol	𝑃	Fold change; Ker → hiPSC	Fold change; hiPSC → Neuron	Fold change hiPSC → CM
Cyclic nucleotide-gated channel alpha 1, transcript variant 2	NM_000087	CNGA1	7.14𝐸−01	1.15
Cyclic nucleotide-gated channel alpha 1, transcript variant 2	NM_000087	CNGA1	𝟏.𝟏𝟖𝐄−𝟎𝟐	𝟐.𝟎𝟎
Cyclic nucleotide-gated channel alpha 3, transcript variant 1	NM_001298	CNGA3	2.00𝐸−01	1.21
Cyclic nucleotide-gated channel alpha 4	NM_001037329	CNGA4	2.78𝐸−01	1.82
Cyclic nucleotide-gated channel beta 1, transcript variant 1	NM_001297	CNGB1	9.22 𝐸−03	−2.05
Cyclic nucleotide-gated channel beta 1, transcript variant 1	NM_001297	CNGB1	6.36𝐸−01	−1.13
Cyclic nucleotide-gated channel beta 3	NM_019098	CNGB3	2.93𝐸−01	1.78

(e) GABA receptors

Gene name	Accession number	Gene symbol	𝑃	Fold change; Ker → hiPSC	Fold change; hiPSC → Neuron	Fold change hiPSC → CM
Gamma-aminobutyric acid (GABA) A receptor, alpha 1, transcript variant 1	NM_000806	GABRA1	1.26𝐸−01	1.41
Gamma-aminobutyric acid (GABA) A receptor, alpha 1, transcript variant 1	NM_000806	GABRA1	8.87𝐸−01	1.05
Gamma-aminobutyric acid (GABA) A receptor, alpha 2, transcript variant 1	NM_000807	GABRA2	9.70𝐸−02	1.70
Gamma-aminobutyric acid (GABA) A receptor, alpha 2, transcript variant 1	NM_000807	GABRA2	4.49𝐸−01	1.48
Gamma-aminobutyric acid (GABA) A receptor, alpha 3	NM_000808	GABRA3	2.10𝐸−01	1.43
Gamma-aminobutyric acid (GABA) A receptor, alpha 4, transcript variant 1	NM_000809	GABRA4	3.76𝐸−01	1.43
Gamma-aminobutyric acid (GABA) A receptor, alpha 4, transcript variant 1	NM_000809	GABRA4	8.30𝐸−01	1.05
Gamma-aminobutyric acid (GABA) A receptor, alpha 5, transcript variant 1	NM_000810	GABRA5	𝟏.𝟎𝟗𝐄−𝟎𝟖	14.41
Gamma-aminobutyric acid (GABA) A receptor, alpha 6	NM_000811	GABRA6	1.69𝐸−01	1.23
Gamma-aminobutyric acid (GABA) A receptor, beta 1	NM_000812	GABRB1	1.14𝐸−01	1.43
Gamma-aminobutyric acid (GABA) A receptor, beta 2, transcript variant 2	NM_000813	GABRB2	1.27𝐸−02	1.94
Gamma-aminobutyric acid (GABA) A receptor, beta 3, transcript variant 1	NM_000814	GABRB3	𝟔.𝟕𝟎𝐄−𝟏𝟎	85.93		−4.49
Gamma-aminobutyric acid (GABA) A receptor, beta 3, transcript variant 1	NM_000814	GABRB3	𝟏.𝟏𝟓𝐄−𝟎𝟗	115.69
Gamma-aminobutyric acid (GABA) A receptor, delta	NM_000815	GABRD	6.26𝐸−02	3.02
Gamma-aminobutyric acid (GABA) A receptor, epsilon	NM_004961	GABRE	5.83 𝐸−05	−5.47
Gamma-aminobutyric acid (GABA) A receptor, Gamma 1	NM_173536	GABRG1	1.78𝐸−01	1.24
Gamma-aminobutyric acid (GABA) A receptor, Gamma 1	NM_173536	GABRG1	𝟏.𝟕𝟎𝐄−𝟎𝟐	2.19
Gamma-aminobutyric acid (GABA) A receptor, Gamma 2, transcript variant 1	NM_198904	GABRG2	6.02𝐸−01	1.14
Gamma-aminobutyric acid (GABA) A receptor, Gamma 2, transcript variant 2	NM_000816	GABRG2	5.28𝐸−02	1.72
Gamma-aminobutyric acid (GABA) A receptor, Gamma 2, transcript variant 2	NM_000816	GABRG2	𝟗.𝟒𝟕𝐄−𝟎𝟗	8.41
Gamma-aminobutyric acid (GABA) A receptor, Gamma 3	NM_033223	GABRG3	1.99𝐸−01	2.00
Gamma-aminobutyric acid (GABA) A receptor, Gamma 3	NM_033223	GABRG3	1.04𝐸−01	1.61
Gamma-aminobutyric acid (GABA) A receptor, pi	NM_014211	GABRP	6.62𝐸−01	−1.12		17.25
Gamma-aminobutyric acid (GABA) receptor, theta	NM_018558	GABRQ	1.60𝐸−01	1.56
Gamma-aminobutyric acid (GABA) receptor, theta	NM_018558	GABRQ	4.76𝐸−01	−1.27
Gamma-aminobutyric acid (GABA) receptor, theta [Source: HGNC Symbol; Acc: 14454]	ENST00000370306	GABRQ	𝟖.𝟐𝟐𝐄−𝟎𝟗	52.17
Gamma-aminobutyric acid (GABA) receptor, rho 1	NM_002042	GABRR1	8.94𝐸−01	−1.04
Gamma-aminobutyric acid (GABA) receptor, rho 2	NM_002043	GABRR2	2.83𝐸−01	1.54
Gamma-aminobutyric acid (GABA) receptor, rho 3	NM_001105580	GABRR3	3.05𝐸−01	−1.34

(f) Glycine receptors

Gene name	Accession number	Gene symbol	𝑃	Fold change; Ker → hiPSC	Fold change; hiPSC → Neuron	Fold change hiPSC → CM
Glycine receptor, alpha 1, transcript variant 2	NM_000171	GLRA1	5.47𝐸−01	−1.29
Glycine receptor, alpha 2, transcript variant 1	NM_002063	GLRA2	1.17𝐸−01	1.42	11.41
Glycine receptor, alpha 3, transcript variant 1	NM_006529	GLRA3	5.16𝐸−01	−1.26
Glycine receptor, alpha 3 [Source: HGNC Symbol; Acc: 4328]	ENST00000274093	GLRA3	8.33𝐸−02	1.64
Glycine receptor, alpha 3, transcript variant 1	NM_006529	GLRA3	1.46𝐸−01	1.41
Glycine receptor, alpha 4, transcript variant 1	NM_001024452	GLRA4	7.75𝐸−01	1.17
Glycine receptor, beta, transcript variant 1	NM_000824	GLRB	6.38𝐸−02	−1.69	6.61

(g) Ionotropic glutamate receptors

Gene name	Accession number	Gene symbol	𝑃	Fold change; Ker → hiPSC	Fold change; hiPSC → Neuron	Fold change hiPSC → CM
Glutamate receptor, ionotropic, AMPA 1, transcript variant 1	NM_000827	GRIA1	7.65𝐸−01	−1.20	2.06
Glutamate receptor, ionotropic, AMPA 2, transcript variant 1	NM_000826	GRIA2	2.87𝐸−01	−1.87	21.26
Glutamate receptor, ionotropic, AMPA 2, transcript variant 1	NM_000826	GRIA2	2.72𝐸−01	1.33
Glutamate receptor, ionotropic, AMPA 2, transcript variant 1	NM_000826	GRIA2	6.54𝐸−01	1.22
Glutamate receptor, ionotropic, AMPA 3, transcript variant 2	NM_000828	GRIA3	5.05𝐸−01	1.26
Glutamate receptor, ionotropic, AMPA 3 [Source: HGNC Symbol; Acc: 4573]	ENST00000371264	GRIA3	1.14𝐸−01	1.50
Glutamate receptor, ionotropic, AMPA 3, transcript variant 2	NM_000828	GRIA3	9.05𝐸−01	−1.05
Glutamate receptor, ionotropic, AMPA 4, transcript variant 1	NM_000829	GRIA4	2.30𝐸−02	1.80	3.73
Glutamate receptor, ionotropic, delta 1	NM_017551	GRID1	1.82𝐸−01	1.50
Glutamate receptor, ionotropic, delta 1	NM_017551	GRID1	𝟓.𝟎𝟖𝐄−𝟎𝟓	3.63
Glutamate receptor, ionotropic, delta 2	NM_001510	GRID2	7.64𝐸−01	−1.11
Glutamate receptor, ionotropic, delta 2	NM_001510	GRID2	𝟓.𝟓𝟓𝐄−𝟎𝟗	55.66
Glutamate receptor, ionotropic, kainate 1, transcript variant 1	NM_000830	GRIK1	1.64𝐸−01	1.29	2.58
Glutamate receptor, ionotropic, kainate 2, transcript variant 3	NM_001166247	GRIK2	1.42𝐸−01	1.56	2.53
Glutamate receptor, ionotropic, kainate 2, transcript variant 1	NM_021956	GRIK2	1.14𝐸−01	−2.01
Glutamate receptor, ionotropic, kainate 2, transcript variant 2	NM_175768	GRIK2	7.80𝐸−01	1.08
Glutamate receptor, ionotropic, kainate 3	NM_000831	GRIK3	1.58𝐸−01	1.64
Glutamate receptor, ionotropic, kainate 3	NM_000831	GRIK3	𝟔.𝟏𝟓𝐄−𝟏𝟎	22.64
Glutamate receptor, ionotropic, kainate 4 [Source: HGNC Symbol; Acc: 4582]	ENST00000527524	GRIK4	𝟏.𝟕𝟕𝐄−𝟏𝟏	18.10
Glutamate receptor, ionotropic, kainate 4	NM_014619	GRIK4	5.73𝐸−02	2.54
Glutamate receptor, ionotropic, kainate 5	NM_002088	GRIK5	2.65𝐸−03	1.79		−2.41
Glutamate receptor, ionotropic, N-methyl D-aspartate 1, transcript variant NR1-3	NM_007327	GRIN1	1.93 𝐸−02	−3.02	2.69
Glutamate receptor, ionotropic, N-methyl D-aspartate 1, transcript variant NR1-3	NM_007327	GRIN1	6.01𝐸−02	−2.45
Glutamate receptor, ionotropic, N-methyl D-aspartate 1, transcript variant NR1-3	NM_007327	GRIN1	3.86𝐸−01	−1.65
Glutamate receptor, ionotropic, N-methyl D-aspartate 2A, transcript variant 2	NM_000833	GRIN2A	6.54𝐸−01	1.18
Glutamate receptor, ionotropic, N-methyl D-aspartate 2A, transcript variant 2	NM_000833	GRIN2A	9.21𝐸−01	−1.02
Glutamate receptor, ionotropic, N-methyl D-aspartate 2A, transcript variant 1	NM_001134407	GRIN2A	𝟑.𝟑𝟐𝐄−𝟏𝟎	29.85
Glutamate receptor, ionotropic, N-methyl D-aspartate 2A, transcript variant 2	NM_000833	GRIN2A	2.63𝐸−01	1.55
Glutamate receptor, ionotropic, N-methyl D-aspartate 2B	NM_000834	GRIN2B	6.64𝐸−01	−1.26
Glutamate receptor, ionotropic, N-methyl D-aspartate 2C	NM_000835	GRIN2C	8.01𝐸−01	−1.08
Glutamate receptor, ionotropic, N-methyl D-aspartate 2C	NM_000835	GRIN2C	5.01𝐸−01	1.13
Glutamate receptor, ionotropic, N-methyl D-aspartate 2D	NM_000836	GRIN2D	4.69𝐸−02	−1.82
Glutamate receptor, ionotropic, N-methyl-D-aspartate 3A	NM_133445	GRIN3A	3.28𝐸−01	−1.41
Glutamate receptor, ionotropic, N-methyl-D-aspartate 3B	NM_138690	GRIN3B	9.36𝐸−01	−1.02
Glutamate receptor, ionotropic, N-methyl D-aspartate-associated protein 1 (glutamate binding), transcript variant 1	NM_000837	GRINA	3.68 𝐸−04	−2.05	2.05
NMDA receptor glutamate-binding chain (hnrgw), partial	U44954	GRINA	3.06𝐸−04	−1.81

(h) Hyperpolarization-activated cyclic nucleotide-gated channels

Gene name	Accession number	Gene symbol	𝑃	Fold change; Ker → hiPSC	Fold change; hiPSC → Neuron	Fold change hiPSC → CM
Hyperpolarization-activated cyclic nucleotide-gated potassium channel 1	NM_021072	HCN1	𝟑.𝟒𝟕𝐄−𝟏𝟏	52.85
Hyperpolarization-activated cyclic nucleotide-gated potassium channel 1	NM_021072	HCN1	𝟏.𝟎𝟏𝐄−𝟎𝟖	9.91
Hyperpolarization-activated cyclic nucleotide-gated potassium channel 2	NM_001194	HCN2	5.36𝐸−02	−1.63
Hyperpolarization-activated cyclic nucleotide-gated potassium channel 3	NM_020897	HCN3	𝟏.𝟗𝟖𝐄−𝟎𝟐	2.12	6.56
Hyperpolarization-activated cyclic nucleotide-gated potassium channel 4	NM_005477	HCN4	1.01𝐸−01	−1.66

(i) Serotonin receptors

Gene name	Accession number	Gene symbol	𝑃	Fold change; Ker → hiPSC	Fold change; hiPSC → Neuron	Fold change hiPSC → CM
5-hydroxytryptamine (serotonin) receptor 3A, transcript variant 1	NM_213621	HTR3A	𝟏.𝟗𝟗𝐄−𝟎𝟑	2.77	−2.20
5-hydroxytryptamine (serotonin) receptor 3A, transcript variant 1	NM_213621	HTR3A	𝟐.𝟔𝟑𝐄−𝟏𝟏	11.11
5-hydroxytryptamine (serotonin) receptor 3B	NM_006028	HTR3B	1.99𝐸−01	−1.54
5-hydroxytryptamine (serotonin) receptor 3, family member C	NM_130770	HTR3C	4.81𝐸−01	1.34
5-hydroxytryptamine (serotonin) receptor 3 family member D, transcript variant 2	NM_182537	HTR3D	4.75𝐸−01	−1.37
5-hydroxytryptamine (serotonin) receptor 3, family member E	NM_182589	HTR3E	1.52𝐸−01	−1.44

(j) Voltage-gated potassium channels

Gene name	Accession number	Gene symbol	𝑃	Fold change; Ker → hiPSC	Fold change; hiPSC → Neuron	Fold change hiPSC → CM
Potassium voltage-gated channel, shaker-related subfamily, member 1 (episodic ataxia with myokymia)	NM_000217	KCNA1	1.64𝐸−01	2.00
Potassium voltage-gated channel, shaker-related subfamily, member 1 (episodic ataxia with myokymia)	NM_000217	KCNA1	1.31𝐸−01	1.87
Potassium voltage-gated channel, shaker-related subfamily, member 2, transcript variant 2	NM_001204269	KCNA2	8.55𝐸−02	1.33
Potassium voltage-gated channel, shaker-related subfamily, member 2, transcript variant 1	NM_004974	KCNA2	8.33𝐸−01	1.06
Potassium voltage-gated channel, shaker-related subfamily, member 3	NM_002232	KCNA3	2.43𝐸−01	1.76
Potassium voltage-gated channel, shaker-related subfamily, member 4	NM_002233	KCNA4	5.75𝐸−01	1.11
Potassium voltage-gated channel, shaker-related subfamily, member 5	NM_002234	KCNA5	𝟏.𝟕𝟔𝐄−𝟎𝟒	4.11		5.63
Potassium voltage-gated channel, shaker-related subfamily, member 6	NM_002235	KCNA6	7.20𝐸−02	2.28
Potassium voltage-gated channel, shaker-related subfamily, member 7	NM_031886	KCNA7	𝟖.𝟓𝟑𝐄−𝟎𝟑	3.67
Potassium voltage-gated channel, shaker-related subfamily, member 7	NM_031886	KCNA7	1.04𝐸−01	2.39
Potassium voltage-gated channel, shaker-related subfamily, member 10	NM_005549	KCNA10	3.32𝐸−01	−1.62
Potassium voltage-gated channel, shaker-related subfamily, beta member 1, transcript variant 2	NM_003471	KCNAB1	2.70𝐸−01	1.46
Potassium voltage-gated channel, shaker-related subfamily, beta member 1, transcript variant 2	NM_003471	KCNAB1	𝟑.𝟔𝟎𝐄−𝟎𝟐	2.42
Potassium voltage-gated channel, shaker-related subfamily, beta member 1, transcript variant 2	NM_003471	KCNAB1	7.87𝐸−02	3.06
Potassium voltage-gated channel, shaker-related subfamily, beta member 2, transcript variant 1	NM_003636	KCNAB2	5.35𝐸−01	−1.35
Potassium voltage-gated channel, shaker-related subfamily, beta member 2, transcript variant 1	NM_003636	KCNAB2	2.20𝐸−01	1.26
Potassium voltage-gated channel, shaker-related subfamily, beta member 3 [Source: HGNC Symbol; Acc: 6230]	ENST00000303790	KCNAB3	8.14𝐸−02	1.62
Potassium voltage-gated channel, shaker-related subfamily, beta member 3	NM_004732	KCNAB3	3.66𝐸−01	1.35
Potassium voltage-gated channel, Shab-related subfamily, member 1	NM_004975	KCNB1	𝟕.𝟒𝟐𝐄−𝟎𝟖	16.32	2.59
Potassium voltage-gated channel, Shab-related subfamily, member 2	NM_004770	KCNB2	𝟏.𝟓𝟒𝐄−𝟎𝟓	4.58
Potassium voltage-gated channel, Shaw-related subfamily, member 1, transcript variant A	NM_001112741	KCNC1	1.47𝐸−01	1.75
Potassium voltage-gated channel, Shaw-related subfamily, member 1, transcript variant B	NM_004976	KCNC1	𝟒.𝟖𝟔𝐄−𝟎𝟖	13.97
Potassium voltage-gated channel, Shaw-related subfamily, member 1, transcript variant A	NM_001112741	KCNC1	2.17𝐸−01	1.56
Potassium voltage-gated channel, Shaw-related subfamily, member 2, transcript variant 1	NM_139136	KCNC2	8.53𝐸−01	1.06
Potassium voltage-gated channel, Shaw-related subfamily, member 2, transcript variant 2	NM_139137	KCNC2	4.04𝐸−01	1.34
Potassium voltage-gated channel, Shaw-related subfamily, member 3	NM_004977	KCNC3	3.56𝐸−01	1.20
Potassium voltage-gated channel, Shaw-related subfamily, member 4, transcript variant 3	NM_001039574	KCNC4	7.70𝐸−02	−1.40
Potassium voltage-gated channel, Shal-related subfamily, member 1	NM_004979	KCND1	𝟏.𝟒𝟖𝐄−𝟎𝟒	7.11
Potassium voltage-gated channel, Shal-related subfamily, member 2	NM_012281	KCND2	𝟏.𝟔𝟐𝐄−𝟏𝟐	91.08	2.76	−2.57
Potassium voltage-gated channel, Shal-related subfamily, member 2	NM_012281	KCND2	𝟐.𝟕𝟔𝐄−𝟎𝟗	21.83
Potassium voltage-gated channel, Shal-related subfamily, member 3 [Source: HGNC Symbol; Acc: 6239]	ENST00000369697	KCND3	5.36𝐸−01	−1.36
Potassium voltage-gated channel, Shal-related subfamily, member 3, transcript variant 1	NM_004980	KCND3	1.05𝐸−01	1.55
Potassium voltage-gated channel, Shal-related subfamily, member 3, transcript variant 1	NM_004980	KCND3	4.41𝐸−01	1.37
Potassium voltage-gated channel, Shal-related subfamily, member 3 [Source: HGNC Symbol; Acc: 6239]	ENST00000369697	KCND3	3.86𝐸−04	−1.84
Potassium voltage-gated channel, Isk-related family, member 1, transcript variant 2	NM_000219	KCNE1	3.53𝐸−01	−1.45
Potassium voltage-gated channel, Isk-related family, member 1, transcript variant 2	NM_000219	KCNE1	3.59𝐸−01	1.25
KCNE1-like	NM_012282	KCNE1L	𝟏.𝟑𝟒𝐄−𝟎𝟖	4.44		−2.18
KCNE1-like	NM_012282	KCNE1L	𝟏.𝟏𝟏𝐄−𝟎𝟖	3.65
KCNE1-like	NM_012282	KCNE1L	6.41𝐸−03	1.88
Potassium voltage-gated channel, Isk-related family, member 2	NM_172201	KCNE2	𝟏.𝟒𝟏𝐄−𝟎𝟒	3.93
Potassium voltage-gated channel, Isk-related family, member 3	NM_005472	KCNE3	𝟖.𝟔𝟓𝐄−𝟏𝟓	52.36
Potassium voltage-gated channel, Isk-related family, member 4	NM_080671	KCNE4	5.04𝐸−01	1.20
Potassium voltage-gated channel, subfamily F, member 1	NM_002236	KCNF1	𝟐.𝟐𝟓𝐄−𝟎𝟓	6.48	2.84
Potassium voltage-gated channel, subfamily G, member 1	NM_002237	KCNG1	8.51𝐸−03	−1.82
Potassium voltage-gated channel, subfamily G, member 1	NM_002237	KCNG1	1.55𝐸−01	−1.65
Potassium voltage-gated channel, subfamily G, member 2	NM_012283	KCNG2	6.21𝐸−02	1.32
Potassium voltage-gated channel, subfamily G, member 3, transcript variant 1	NM_133329	KCNG3	𝟑.𝟕𝟒𝐄−𝟏𝟏	58.95		−2.93
Potassium voltage-gated channel, subfamily G, member 4	NM_172347	KCNG4	3.36 𝐸−03	−3.33
Potassium voltage-gated channel, subfamily G, member 4, (cDNA clone IMAGE: 3028985)	BC008969	KCNG4	2.24 𝐸−04	−3.29
Potassium voltage-gated channel, subfamily H (eag-related), member 1, transcript variant 1	NM_172362	KCNH1	8.05𝐸−01	−1.15
Potassium voltage-gated channel, subfamily H (eag-related), member 2, transcript variant 1	NM_000238	KCNH2	𝟓.𝟗𝟒𝐄−𝟎𝟒	37.53
Potassium voltage-gated channel, subfamily H (eag-related), member 2, transcript variant 2	NM_172056	KCNH2	5.70𝐸−03	1.73
Potassium voltage-gated channel, subfamily H (eag-related), member 3	NM_012284	KCNH3	9.65𝐸−01	1.01
Potassium voltage-gated channel, subfamily H (eag-related), member 4	NM_012285	KCNH4	3.18𝐸−01	−1.24
Potassium voltage-gated channel, subfamily H (eag-related), member 5, transcript variant 2	NM_172376	KCNH5	𝟑.𝟎𝟒𝐄−𝟎𝟑	2.96
Potassium voltage-gated channel, subfamily H (eag-related), member 5, transcript variant 1	NM_139318	KCNH5	5.41𝐸−01	1.47
Potassium voltage-gated channel, subfamily H (eag-related), member 6, transcript variant 2	NM_173092	KCNH6	𝟐.𝟗𝟑𝐄−𝟎𝟐	3.04
cDNA FLJ33650 fis, clone BRAMY2024514, highly similar to Rattus norvegicus Potassium channel (erg2)	AK090969	KCNH6	𝟐.𝟎𝟖𝐄−𝟎𝟓	12.42
Potassium voltage-gated channel, subfamily H (eag-related), member 6, transcript variant 2	NM_173092	KCNH6	𝟐.𝟓𝟓𝐄−𝟎𝟐	2.29
Potassium voltage-gated channel, subfamily H (eag-related), member 7, transcript variant 2	NM_173162	KCNH7	4.63𝐸−01	−1.40
Potassium voltage-gated channel, subfamily H (eag-related), member 7, transcript variant 1	NM_033272	KCNH7	7.58𝐸−01	−1.09
Potassium voltage-gated channel, subfamily H (eag-related), member 8	NM_144633	KCNH8	𝟑.𝟎𝟎𝐄−𝟎𝟗	24.57	2.97
Kv channel interacting protein 1, transcript variant 1	NM_001034837	KCNIP1	8.33𝐸−02	1.96	4.24
Kv channel interacting protein 2, transcript variant 7	NM_173197	KCNIP2	6.43𝐸−01	1.21
Kv channel interacting protein 2, transcript variant 1	NM_014591	KCNIP2	2.53𝐸−03	1.96
Kv channel interacting protein 3, calsenilin, transcript variant 1	NM_013434	KCNIP3	1.15 𝐸−03	−3.47
Kv channel interacting protein 4, transcript variant 5	NM_001035003	KCNIP4	6.73𝐸−01	1.16		2.01
Kv channel interacting protein 4, transcript variant 5	NM_001035003	KCNIP4	9.26𝐸−01	1.05
Potassium voltage-gated channel, KQT-like subfamily, member 1, transcript variant 1	NM_000218	KCNQ1	𝟑.𝟑𝟏𝐄−𝟎𝟗	79.52
Potassium voltage-gated channel, KQT-like subfamily, member 2, transcript variant 5	NM_172109	KCNQ2	𝟓.𝟔𝟖𝐄−𝟏𝟗	3408.43
Potassium voltage-gated channel, KQT-like subfamily, member 2, transcript variant 3	NM_004518	KCNQ2	1.29𝐸−02	1.82
Potassium voltage-gated channel, KQT-like subfamily, member 2, (cDNA clone IMAGE: 4154700)	BC020384	KCNQ2	𝟏.𝟎𝟗𝐄−𝟎𝟖	37.12
Potassium voltage-gated channel, KQT-like subfamily, member 3, transcript variant 1	NM_004519	KCNQ3	1.10𝐸−04	−1.69
Potassium voltage-gated channel, KQT-like subfamily, member 4, transcript variant 1	NM_004700	KCNQ4	9.77𝐸−01	1.02
Potassium voltage-gated channel, KQT-like subfamily, member 5, transcript variant 1	NM_019842	KCNQ5	9.81𝐸−02	−1.79
Potassium voltage-gated channel, delayed-rectifier, subfamily S, member 1	NM_002251	KCNS1	3.39𝐸−01	−1.27		−2.64
Potassium voltage-gated channel, delayed-rectifier, subfamily S, member 2	NM_020697	KCNS2	6.19𝐸−02	−2.45	2.11
Potassium voltage-gated channel, delayed-rectifier, subfamily S, member 3	NM_002252	KCNS3	𝟏.𝟎𝟒𝐄−𝟎𝟒	2.22	−2.61	−2.39
Potassium channel, subfamily T, member 1 (sodium activated)	NM_020822	KCNT1	8.51𝐸−02	−2.17
Potassium channel, subfamily V, member 1	NM_014379	KCNV1	7.84𝐸−02	1.58
Potassium channel, subfamily V, member 2	NM_133497	KCNV2	7.63𝐸−01	−1.16

(k) Inwardly rectifying potassium channels

Gene name	Accession number	Gene symbol	𝑃	Fold change; Ker → hiPSC	Fold change; hiPSC → Neuron	Fold change hiPSC → CM
Potassium inwardly-rectifying channel, subfamily J, member 1, transcript variant rom-k5	NM_153767	KCNJ1	𝟐.𝟓𝟒𝐄−𝟎𝟐	2.66
Potassium inwardly-rectifying channel, subfamily J, member 1, transcript variant rom-k5	NM_153767	KCNJ1	8.51𝐸−01	−1.05
Potassium inwardly-rectifying channel, subfamily J, member 2	NM_000891	KCNJ2	𝟓.𝟏𝟒𝐄−𝟎𝟒	4.38
Potassium inwardly-rectifying channel, subfamily J, member 3	NM_002239	KCNJ3	4.56𝐸−01	1.34
Potassium inwardly-rectifying channel, subfamily J, member 4, transcript variant 1	NM_152868	KCNJ4	𝟒.𝟖𝟎𝐄−𝟎𝟐	2.30
Potassium inwardly-rectifying channel, subfamily J, member 5	NM_000890	KCNJ5	1.88 𝐸−08	−45.40
Potassium inwardly-rectifying channel, subfamily J, member 5	NM_000890	KCNJ5	4.42 𝐸−12	−109.11
Potassium inwardly-rectifying channel, subfamily J, member 6	NM_002240	KCNJ6	𝟑.𝟐𝟗𝐄−𝟎𝟓	6.75	5.54
Potassium inwardly-rectifying channel, subfamily J, member 8	NM_004982	KCNJ8	𝟏.𝟗𝟖𝐄−𝟎𝟒	5.97		2.74
Potassium inwardly-rectifying channel, subfamily J, member 9 [Source: HGNC Symbol; Acc: 6270]	ENST00000368088	KCNJ9	3.17𝐸−01	1.49
Potassium inwardly-rectifying channel, subfamily J, member 9	NM_004983	KCNJ9	3.07𝐸−01	1.79
Potassium inwardly-rectifying channel, subfamily J, member 10	NM_002241	KCNJ10	𝟖.𝟖𝟓𝐄−𝟎𝟒	4.14
Potassium inwardly-rectifying channel, subfamily J, member 10	NM_002241	KCNJ10	9.56𝐸−01	1.01
Potassium inwardly-rectifying channel, subfamily J, member 11, transcript variant 1	NM_000525	KCNJ11	𝟒.𝟎𝟎𝐄−𝟎𝟒	2.93
Potassium inwardly-rectifying channel, subfamily J, member 12	NM_021012	KCNJ12	1.00𝐸−01	1.47
Potassium inwardly-rectifying channel, subfamily J, member 12	NM_021012	KCNJ12	𝟑.𝟓𝟑𝐄−𝟎𝟔	5.81
Potassium inwardly-rectifying channel, subfamily J, member 13, transcript variant 1	NM_002242	KCNJ13	7.75𝐸−01	1.20
Potassium inwardly-rectifying channel, subfamily J, member 14, transcript variant 2	NM_170720	KCNJ14	9.05𝐸−02	−1.40
Potassium inwardly-rectifying channel, subfamily J, member 15, transcript variant 1	NM_170736	KCNJ15	3.41 𝐸−08	−150.95
Potassium inwardly-rectifying channel, subfamily J, member 16, transcript variant 2	NM_170741	KCNJ16	2.22𝐸−01	−1.93

(l) Two-P potassium channels

Gene name	Accession number	Gene symbol	𝑃	Fold change; Ker → hiPSC	Fold change; hiPSC → Neuron	Fold change hiPSC → CM
Potassium channel, subfamily K, member 1	NM_002245	KCNK1	3.02𝐸−04	−1.68
Potassium channel, subfamily K, member 2, transcript variant 1	NM_001017424	KCNK2	3.46𝐸−01	1.57
Potassium channel, subfamily K, member 3	NM_002246	KCNK3	9.98𝐸−01	1.00	2.36
Potassium channel, subfamily K, member 3	NM_002246	KCNK3	8.75𝐸−01	−1.10
Potassium channel, subfamily K, member 3	NM_002246	KCNK3	5.34𝐸−01	−1.30
Potassium channel, subfamily K, member 3 [Source: HGNC Symbol; Acc: 6278]	ENST00000302909	KCNK3	2.53𝐸−01	1.52
Potassium channel, subfamily K, member 4	NM_033310	KCNK4	6.21𝐸−01	−1.14	2.05
Potassium channel, subfamily K, member 5	NM_003740	KCNK5	𝟏.𝟏𝟔𝐄−𝟏𝟒	29.46	−2.35	−2.03
Potassium channel, subfamily K, member 6	NM_004823	KCNK6	1.22 𝐸−06	−3.48	−2.20
Potassium channel, subfamily K, member 6	NM_004823	KCNK6	2.05 𝐸−08	−5.11
Potassium channel, subfamily K, member 6	NM_004823	KCNK6	7.04E−06	−5.22
Potassium channel, subfamily K, member 7, transcript variant A	NM_033347	KCNK7	1.17𝐸−01	−1.52
Potassium channel, subfamily K, member 9	NM_016601	KCNK9	6.67𝐸−01	−1.29
Potassium channel, subfamily K, member 10, transcript variant 1	NM_021161	KCNK10	2.63𝐸−01	−1.30
Potassium channel, subfamily K, member 10, transcript variant 2	NM_138317	KCNK10	1.41𝐸−02	1.43
Potassium channel, subfamily K, member 12	NM_022055	KCNK12	𝟒.𝟓𝟖𝐄−𝟏𝟎	23.40		−5.58
Potassium channel, subfamily K, member 13	NM_022054	KCNK13	7.65𝐸−01	−1.10
Potassium channel, subfamily K, member 15	NM_022358	KCNK15	4.96𝐸−02	−1.50
Potassium channel, subfamily K, member 15	NM_022358	KCNK15	9.10𝐸−01	−1.09
Pancreatic potassium channel TALK-1d; alternatively spliced	AY253147	KCNK16	2.17 𝐸−02	−2.43
Potassium channel, subfamily K, member 17, transcript variant 1	NM_031460	KCNK17	𝟏.𝟖𝟕𝐄−𝟏𝟏	28.60
Potassium channel, subfamily K, member 17, transcript variant 1	NM_031460	KCNK17	𝟏.𝟓𝟐𝐄−𝟎𝟓	19.62
Potassium channel, subfamily K, member 18	NM_181840	KCNK18	1.77𝐸−01	1.61

(m) Calcium-activated potassium channels

Gene name	Accession number	Gene symbol	𝑃	Fold change; Ker → hiPSC	Fold change; hiPSC → Neuron	Fold change hiPSC → CM
Potassium large conductance calcium-activated channel, subfamily M, alpha member 1, transcript variant 2	NM_002247	KCNMA1	1.01𝐸−02	1.84		2.01
Potassium large conductance calcium-activated channel, subfamily M, alpha member 1, transcript variant 1	NM_001014797	KCNMA1	3.19𝐸−01	1.32
Potassium large conductance calcium-activated channel, subfamily M, alpha member 1, transcript variant 2	NM_002247	KCNMA1	𝟑.𝟔𝟕𝐄−𝟎𝟑	2.27
Potassium large conductance calcium-activated channel, subfamily M, alpha member 1, transcript variant 2	NM_002247	KCNMA1	8.53𝐸−02	1.44
Maxi-K channel HSLO	AF349445	KCNMA1	3.04𝐸−01	1.44
Potassium large conductance calcium-activated channel, subfamily M, alpha member 1, transcript variant 2	NM_002247	KCNMA1	2.15𝐸−01	−1.23
Potassium large conductance calcium-activated channel, subfamily M, beta member 1	NM_004137	KCNMB1	7.12𝐸−02	−1.41
Potassium large conductance calcium-activated channel, subfamily M, beta member 1	NM_004137	KCNMB1	𝟐.𝟐𝟑𝐄−𝟎𝟔	7.82
Potassium large conductance calcium-activated channel, subfamily M, beta member 2, transcript variant 1	NM_181361	KCNMB2	3.36𝐸−01	1.56
Potassium large conductance calcium-activated channel, subfamily M, beta member 2, transcript variant 1	NM_181361	KCNMB2	1.17𝐸−01	1.47
Potassium large conductance calcium-activated channel, subfamily M beta member 3, transcript variant 1	NM_171828	KCNMB3	𝟏.𝟒𝟏𝐄−𝟎𝟓	2.63
Potassium large conductance calcium-activated channel, subfamily M, beta member 4	NM_014505	KCNMB4	𝟖.𝟖𝟓𝐄−𝟏𝟐	103.99
Potassium large conductance calcium-activated channel, subfamily M, beta member 4 [Source: HGNC Symbol; Acc: 6289]	ENST00000258111	KCNMB4	𝟗.𝟐𝟐𝐄−𝟏𝟎	18.40
Potassium intermediate/small conductance calcium-activated channel, subfamily N, member 1	NM_002248	KCNN1	𝟑.𝟒𝟎𝐄−𝟎𝟓	4.67
Potassium intermediate/small conductance calcium-activated channel, subfamily N, member 2, transcript variant 1	NM_021614	KCNN2	𝟏.𝟖𝟖𝐄−𝟏𝟑	22.23	−3.63
Potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3, transcript variant 1	NM_002249	KCNN3	𝟐.𝟓𝟖𝐄−𝟎𝟔	8.25
Potassium intermediate/small conductance calcium-activated channel, subfamily N, member 3, transcript variant 3	NM_001204087	KCNN3	𝟏.𝟏𝟏𝐄−𝟎𝟕	15.29
Potassium intermediate/small conductance calcium-activated channel, subfamily N, member 4	NM_002250	KCNN4	1.06𝐸−02	−1.84

(n) P2X receptors

Gene name	Accession number	Gene symbol	𝑃	Fold change; Ker → hiPSC	Fold change; hiPSC → Neuron	Fold change hiPSC → CM
Purinergic receptor P2X, ligand-gated ion channel, 1	NM_002558	P2RX1	7.71𝐸−01	1.14
Purinergic receptor P2X, ligand-gated ion channel, 2, transcript variant 4	NM_170683	P2RX2	5.25𝐸−02	−1.66
Purinergic receptor P2X, ligand-gated ion channel, 3	NM_002559	P2RX3	2.43𝐸−02	−1.76
Purinergic receptor P2X, ligand-gated ion channel, 4	NM_002560	P2RX4	8.68𝐸−01	−1.03
Purinergic receptor P2X, ligand-gated ion channel, 5, transcript variant 1	NM_002561	P2RX5	𝟕.𝟔𝟖𝐄−𝟎𝟖	6.97
Purinergic receptor P2X, ligand-gated ion channel, 5, transcript variant 2	NM_175080	P2RX5	6.13𝐸−02	1.54
Purinergic receptor P2X, ligand-gated ion channel, 6 [Source: HGNC Symbol; Acc: 8538]	ENST00000413302	P2RX6	4.13𝐸−01	1.68
Purinergic receptor P2X, ligand-gated ion channel, 7, transcript variant 1	NM_002562	P2RX7	6.13𝐸−01	1.23
Purinergic receptor P2X, ligand-gated ion channel, 7, transcript variant 1	NM_002562	P2RX7	9.17𝐸−01	1.04

(o) Transient receptor potential channels

Gene name	Accession number	Gene symbol	𝑃	Fold change; Ker → hiPSC	Fold change; hiPSC → Neuron	Fold change hiPSC → CM
Polycystic kidney disease 2 (autosomal dominant)	NM_000297	PKD2	7.48𝐸−07	1.79
Polycystic kidney disease 2 (autosomal dominant)	NM_000297	PKD2	2.38𝐸−05	1.98
Polycystic kidney disease 2-like 1	NM_016112	PKD2L1	𝟏.𝟐𝟗𝐄−𝟎𝟑	4.66
Polycystic kidney disease 2-like 2	NM_014386	PKD2L2	1.94𝐸−01	1.49
Polycystic kidney disease 2-like 2	NM_014386	PKD2L2	7.43𝐸−01	1.19
Polycystic kidney disease 2-like 2	NM_014386	PKD2L2	6.74𝐸−01	1.09
Transient receptor potential cation channel, subfamily A, member 1	NM_007332	TRPA1	2.99𝐸−01	1.74
Transient receptor potential cation channel, subfamily A, member 1	NM_007332	TRPA1	5.64𝐸−02	2.70
Transient receptor potential cation channel, subfamily C, member 1	NM_003304	TRPC1	𝟒.𝟑𝟎𝐄−𝟎𝟔	2.01
Transient receptor potential cation channel, subfamily C, member 3, transcript variant 2	NM_003305	TRPC3	𝟏.𝟏𝟐𝐄−𝟎𝟕	5.78		2.45
Transient receptor potential cation channel, subfamily C, member 4, transcript variant alpha	NM_016179	TRPC4	𝟑.𝟐𝟓𝐄−𝟎𝟕	25.38
Transient receptor potential cation channel, subfamily C, member 5	NM_012471	TRPC5	1.20𝐸−01	1.28
Transient receptor potential cation channel, subfamily C, member 6	NM_004621	TRPC6	1.21𝐸−01	2.33
Transient receptor potential cation channel, subfamily C, member 7, transcript variant 1	NM_020389	TRPC7	6.18𝐸−01	−1.36
Transient receptor potential cation channel, subfamily M, member 1	NM_002420	TRPM1	2.29𝐸−01	1.31
Transient receptor potential cation channel, subfamily M, member 2, transcript variant 1	NM_003307	TRPM2	1.22𝐸−01	1.97
Transient receptor potential cation channel, subfamily M, member 2, transcript variant 1	NM_003307	TRPM2	5.04𝐸−02	2.53
Transient receptor potential cation channel, subfamily M, member 3, transcript variant 7	NM_206948	TRPM3	𝟑.𝟎𝟒𝐄−𝟎𝟗	10.69
Transient receptor potential cation channel, subfamily M, member 3 [Source: HGNC; Symbol; Acc: 17992]	ENST00000354500	TRPM3	𝟐.𝟑𝟒𝐄−𝟎𝟑	3.74
Transient receptor potential cation channel, subfamily M, member 3, transcript variant 7	NM_206948	TRPM3	𝟑.𝟒𝟏𝐄−𝟎𝟔	6.23
Transient receptor potential cation channel, subfamily M, member 3, transcript variant 9	NM_001007471	TRPM3	7.23𝐸−01	1.13
Transient receptor potential cation channel, subfamily M, member 3, transcript variant 9	NM_001007471	TRPM3	𝟏.𝟓𝟒𝐄−𝟎𝟓	2.60
Transient receptor potential cation channel, subfamily M, member 4, transcript variant 1	NM_017636	TRPM4	3.72𝐸−02	−1.89
Transient receptor potential cation channel, subfamily M, member 5	NM_014555	TRPM5	8.21𝐸−01	−1.08
Transient receptor potential cation channel, subfamily M, member 6, transcript variant a	NM_017662	TRPM6	3.80𝐸−01	1.64
Transient receptor potential cation channel, subfamily M, member 6, transcript variant a	NM_017662	TRPM6	9.10𝐸−01	1.05
Transient receptor potential cation channel, subfamily M, member 6, transcript variant a	NM_017662	TRPM6	𝟓.𝟑𝟐𝐄−𝟎𝟕	5.59
Transient receptor potential cation channel, subfamily M, member 6, transcript variant a	NM_017662	TRPM6	3.32𝐸−01	1.36
Transient receptor potential cation channel, subfamily M, member 7	NM_017672	TRPM7	2.16𝐸−02	−1.25
Transient receptor potential cation channel, subfamily M, member 7	NM_017672	TRPM7	3.64𝐸−03	−1.38
Transient receptor potential cation channel, subfamily M, member 8	NM_024080	TRPM8	2.04𝐸−01	1.89
Transient receptor potential cation channel, subfamily V, member 1, transcript variant 3	NM_080706	TRPV1	3.59𝐸−05	1.92
Transient receptor potential cation channel, subfamily V, member 1, transcript variant 3	NM_080706	TRPV1	𝟒.𝟏𝟒𝐄−𝟎𝟓	2.25
Transient receptor potential cation channel, subfamily V, member 2	NM_016113	TRPV2	𝟖.𝟎𝟐𝐄−𝟎𝟖	4.68
Transient receptor potential cation channel, subfamily V, member 3	NM_145068	TRPV3	4.64𝐸−01	1.35
Transient receptor potential cation channel, subfamily V, member 3	NM_145068	TRPV3	1.35𝐸−01	−2.39
Transient receptor potential cation channel, subfamily V, member 4, transcript variant 2	NM_147204	TRPV4	4.33𝐸−01	1.39
Transient receptor potential cation channel, subfamily V, member 5, (cDNA clone MGC: 34269 IMAGE: 5186668)	BC034740	TRPV5	1.00𝐸−01	1.38
Transient receptor potential cation channel, subfamily V, member 5	NM_019841	TRPV5	3.14𝐸−01	−1.40
Transient receptor potential cation channel, subfamily V, member 6	NM_018646	TRPV6	1.57𝐸−01	−1.38

(p) Voltage-gated sodium channels

Gene name	Accession number	Gene symbol	𝑃	Fold change; Ker → hiPSC	Fold change; hiPSC → Neuron	Fold change hiPSC → CM
Sodium channel, voltage-gated, type I, alpha subunit, transcript variant 2	NM_006920	SCN1A	1.98𝐸−01	1.54
Sodium channel, voltage-gated, type I, beta, transcript variant b	NM_199037	SCN1B	9.95𝐸−01	−1.00
Sodium channel, voltage-gated, type II, alpha subunit, transcript variant 1	NM_021007	SCN2A	4.16𝐸−01	1.62	5.00
Sodium channel, voltage-gated, type II, beta	NM_004588	SCN2B	3.61𝐸−01	−1.58
Sodium channel, voltage-gated, type II, beta	NM_004588	SCN2B	8.16 𝐸−04	−2.41
Sodium channel, voltage-gated, type III, alpha subunit, transcript variant 1	NM_006922	SCN3A	2.87𝐸−01	−2.00	2.85
Sodium channel, voltage-gated, type III, alpha subunit, transcript variant 1	NM_006922	SCN3A	1.89𝐸−01	1.49
Sodium channel, voltage-gated, type III, beta, transcript variant 1	NM_018400	SCN3B	8.74𝐸−01	−1.06	4.70
Sodium channel, voltage-gated, type III, beta, transcript variant 1	NM_018400	SCN3B	1.57𝐸−01	−1.46
Sodium channel, voltage-gated, type III, beta, transcript variant 1	NM_018400	SCN3B	1.56𝐸−01	1.96
Sodium channel, voltage-gated, type IV, alpha subunit	NM_000334	SCN4A	𝟏.𝟔𝟐𝐄−𝟎𝟖	9.91
Sodium channel, voltage-gated, type IV, beta, transcript variant 1	NM_174934	SCN4B	3.25 𝐸−04	−3.66
Sodium channel, voltage-gated, type V, alpha subunit, transcript variant 1	NM_198056	SCN5A	𝟐.𝟒𝟑𝐄−𝟎𝟖	14.10
Sodium channel, voltage-gated, type V, alpha subunit, transcript variant 2	NM_000335	SCN5A	4.93𝐸−02	1.95
Sodium channel, voltage-gated, type VII, alpha	NM_002976	SCN7A	4.49𝐸−03	1.63
Sodium channel, voltage-gated, type VII, alpha	NM_002976	SCN7A	2.07𝐸−01	1.72
Sodium channel, voltage gated, type VIII, alpha subunit, transcript variant 1	NM_014191	SCN8A	𝟏.𝟖𝟗𝐄−𝟎𝟗	15.61
Sodium channel, voltage gated, type VIII, alpha subunit, transcript variant 1	NM_014191	SCN8A	𝟒.𝟎𝟔𝐄−𝟎𝟕	11.23
Sodium channel, voltage-gated, type IX, alpha subunit	NM_002977	SCN9A	𝟒.𝟎𝟖𝐄−𝟎𝟖	11.51
Sodium channel, voltage-gated, type IX, alpha subunit	NM_002977	SCN9A	1.62𝐸−01	1.66
Sodium channel, voltage-gated, type IX, alpha subunit	NM_002977	SCN9A	𝟑.𝟑𝟖𝐄−𝟎𝟑	2.57
Sodium channel, voltage-gated, type X, alpha subunit	NM_006514	SCN10A	4.06𝐸−01	1.35
Sodium channel, voltage-gated, type XI, alpha subunit	NM_014139	SCN11A	3.02𝐸−01	1.57
Sodium channel, voltage-gated, type XI, alpha subunit [Source: HGNC Symbol; Acc: 10583]	ENST00000444237	SCN11A	8.83𝐸−03	1.94

(q) Nonvoltage-gated sodium channels

Gene name	Accession number	Gene symbol	𝑃	Fold change; Ker → hiPSC	Fold change; hiPSC → Neuron	Fold change hiPSC → CM
Sodium channel, nonvoltage-gated 1 alpha, transcript variant 1	NM_001038	SCNN1A	𝟏.𝟑𝟑𝐄−𝟎𝟗	51.49	−21.13	−10.71
Sodium channel, nonvoltage-gated 1, beta	NM_000336	SCNN1B	2.11𝐸−01	1.64
Sodium channel, nonvoltage-gated 1, beta	NM_000336	SCNN1B	5.52𝐸−01	1.09
Sodium channel, nonvoltage-gated 1, delta, transcript variant 1	NM_001130413	SCNN1D	8.43𝐸−02	1.49
Sodium channel, nonvoltage-gated 1, gamma	NM_001039	SCNN1G	2.33𝐸−01	1.59
Gamma subunit of epithelial amiloride-sensitive sodium channel	X87160	SCNN1G	3.99𝐸−01	1.27

(r) Two-pore channels

Gene name	Accession number	Gene symbol	𝑃	Fold change; Ker → hiPSC	Fold change; hiPSC → Neuron	Fold change hiPSC → CM
Two-pore segment channel 1, transcript variant 1	NM_001143819	TPCN1	6.20𝐸−04	−1.76
Two-pore segment channel 1, transcript variant 2	NM_017901	TPCN1	1.71 𝐸−03	−2.10
Two-pore segment channel 1, transcript variant 2	NM_017901	TPCN1	3.55 𝐸−04	−2.44
Two-pore segment channel 1, transcript variant 1	NM_001143819	TPCN1	8.06𝐸−03	−1.83
Two-pore segment channel 2	NM_139075	TPCN2	5.06𝐸−01	−1.15

(s) Zinc-activated ligand-gated ion channels

Gene name	Accession number	Gene symbol	𝑃	Fold change; Ker → hiPSC	Fold change; hiPSC → Neuron	Fold change hiPSC → CM
Zinc-activated ligand-gated ion channel	NM_180990	ZACN	9.24𝐸−02	1.45