Research Article

The Assessment of Parameters Affecting the Quality of Cord Blood by the Appliance of the Annexin V Staining Method and Correlation with CFU Assays

Figure 1

(a) Staining with solely 7-AAD, as in the ISHAGE protocol, showed only low amounts of dead CD3 cells following the volume reduction as well as thawing. ((b), (c)) Combination of 7-AAD with Annexin V revealed already remarkable amounts of apoptotic cells in processed samples ahead of cryopreservation which increases furthermore after thawing. Only cells negative for 7-AAD as well as Annexin V could be regarded as fully viable.