Wnt1 Accelerates an Ex Vivo Expansion of Human Cord Blood CD34⁺CD38⁻ Cells

Figure 5

Pluripotency genes expression. Relative quantitation of (a) c-myc, (b) nanog, (c) oct3/4, and (d) sox2 genes expression analyzed by real-time RT-PCR of expanded CB-CD34⁺ cells cultured in 4F and 5F in KSR compared with unexpanded cells (D0). The glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a house keeping gene control. Data shown are mean ± SD of three experiments. *𝑃<0.001, **𝑃<0.02.

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