Improved Method for Ex Ovo-Cultivation of Developing Chicken Embryos for Human Stem Cell Xenografts

Figure 1

Schematic view of initial steps in ex ovo-cultivation of chicken embryos. Prior to transfer, chicken embryos were incubated at 37.8°C for 48 hours. Eggs were gently opened using a jigsaw. The indentation was expanded, and the contents were carefully transferred onto the support film. Ground eggshell of several eggs was added to the albumin and the experimental setup was covered using a bacterial dish.