Stem Cells International / 2013 / Article / Fig 3

Research Article

Increased Haematopoietic Supportive Function of USSC from Umbilical Cord Blood Compared to CB MSC and Possible Role of DLK-1

Figure 3

Regulations of DLK-1 in human and mice are not the same. To evaluate mechanisms induced by DLK-1 in human CB MSC upon exposure to DLK-1/Pref1, Western Blot analysis of ERK1/2 and p-ERK1/2 was performed ( experiments). CB MSC were treated with conditioned media of either the control cells or the DLK-1 overexpressing CB MSC. CB MSC (d0 control) and DLK-1 overexpressing CB MSC (d0 DLK+) were lysed and western blot analysis was performed applying full protein lysates after 30 minutes, 4 hours and 24 hours of incubation with conditioned media, respectively. ERK1/2 was already highly expressed in the non treated cells and remained expressed also in the cells treated. No DLK-1/Pref1 specific upregulation of p-ERK1/2 was detected in the CB MSC. As biological positive control, osteogenic differentiated (day 3 after induction) BM MSC were used. Internal loading control: -actin.

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