Fibroblast Growth Factor 18 Increases the Trophic Effects of Bone Marrow Mesenchymal Stem Cells on Chondrocytes Isolated from Late Stage Osteoarthritic Patients

Figure 2

Expression of chondrogenic genes was measured by qPCR in OA chondrocytes cultured in transwells. (a) Diagram shows how monoculture or coculture is performed in transwell systems. (b) Expression of chondrogenic genes in chondrocytes cultured in transwells in medium without FGF18. Beta-actin was used for normalization. Monoculture was chosen as reference. Number in coculture represents the relative expression level of corresponding gene comparing to monoculture. Three donor pairs were analyzed. NS: nonsignificance. (c) Expression of chondrogenic genes in chondrocytes cultured in transwells in medium containing FGF18 (10 ng/mL). Beta-actin was used for normalization. Monoculture was chosen as reference. Number in coculture represents the relative expression level of corresponding gene comparing to monoculture. Three donor pairs were analyzed. values are calculated with Student’s -test.

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