A Cocktail Method for Promoting Cardiomyocyte Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells

<table>mRNA and protein expressions of beta-catenin, cTnT, and GSK-3beta detected by qPCR and Western blotting in MSCs. (a) Beta-catenin mRNA expression; (b) cTnT mRNA expression; (c) GSK-3beta mRNA expression; (d) protein expression of beta-catenin, cTnT, and GSK-3beta was detected by Western blotting. The group sequence from left to right is control group, 5-aza, SalB, 5-aza+SalB, CLM, 5-aza+CLM, SalB+CLM, and 5-aza+SalB+CLM. Results are shown as the mean ± SD values. Experiments were repeated three times. <svg height="17.825001" id="M2" style="vertical-align:-3.13504pt" version="1.1" viewbox="0 0 69.150002 17.825001" width="69.150002" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg> compared to the control group. <svg height="17.825001" id="M3" style="vertical-align:-3.13504pt" version="1.1" viewbox="0 0 76.137497 17.825001" width="76.137497" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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q-4 71 -28 134q17 33 42 46q24 -12 42 -46q-24 -63 -28 -134q36 23 56.5 41.5t45.5 49.5q36 2 61 -13q0 -28 -19 -59q-65 -10 -130 -43q65 -33 130 -43q19 -31 19 -59z" id="x2217"></path></g><g transform="matrix(.012,-0,0,-.012,9.885,5.525)"><path d="M471 153q-22 -15 -61 -13q-25 31 -45.5 49.5t-56.5 41.5q4 -71 28 -134q-17 -33 -42 -46q-24 12 -42 46q24 63 28 134q-36 -23 -56.5 -41.5t-45.5 -49.5q-36 -2 -61 13q0 28 19 59q65 10 130 43q-65 33 -130 43q-19 31 -19 59q22 15 61 13q25 -31 45.5 -49.5t56.5 -41.5
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</svg> compared between the two groups as shown in the figure.</table>

Stem Cells International

fig4

Figure 4

Figure 4: A Cocktail Method for Promoting Cardiomyocyte Differentiation from Bone Marrow-Derived Mesenchymal Stem Cells 